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Product Info Summary
| SKU: | A05314-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Forkhead box protein E3 FoxE3 Antibody
SKU/Catalog Number
A05314-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Forkhead box protein E3 FoxE3 Antibody catalog # A05314-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Forkhead box protein E3 FoxE3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05314-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FOXE3. AA range:81-130
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05314-1 is reactive to FOXE3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
33234 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05314-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562 cells using FoxE3 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For FOXE3 (Source: Uniprot.org, NCBI)
Gene Name
FOXE3
Full Name
Forkhead box protein E3
Weight
33234 MW
Alternative Names
Forkhead box protein E3;Forkhead-related protein FKHL12;Forkhead-related transcription factor 8;FREAC-8;FOXE3;FKHL12, FREAC8; FOXE3 AAT11, ASGD2, CTRCT34, FKHL12, FREAC8 forkhead box E3 forkhead box protein E3|FREAC-8|forkhead, drosophila, homolog-like 12|forkhead-related protein FKHL12|forkhead-related transcription factor 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXE3, check out the FOXE3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXE3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Forkhead box protein E3 FoxE3 Antibody (A05314-1)
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No publications found for A05314-1
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Customer Q&As
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7 Customer Q&As for Anti-Forkhead box protein E3 FoxE3 Antibody
Question
Is this A05314-1 anti-FoxE3 antibody reactive to the isotypes of FOXE3?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
The immunogen of A05314-1 anti-FoxE3 antibody is Synthesized peptide derived from the Internal region of human FoxE3. at AA rangle: 50-130. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-18
Question
Will anti-FoxE3 antibody A05314-1 work for WB with lens epithelium?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
According to the expression profile of lens epithelium, FOXE3 is highly expressed in lens epithelium. So, it is likely that anti-FoxE3 antibody A05314-1 will work for WB with lens epithelium.
Boster Scientific Support
Answered: 2020-02-05
Question
My question regarding product A05314-1, anti-FoxE3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05314-1 anti-FoxE3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-05
Question
I see that the anti-FoxE3 antibody A05314-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-16
Question
We are currently using anti-FoxE3 antibody A05314-1 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
The anti-FoxE3 antibody (A05314-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-11
Question
Please see the WB image, lot number and protocol we used for lens epithelium using anti-FoxE3 antibody A05314-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-03
Question
Is a blocking peptide available for product anti-FoxE3 antibody (A05314-1)?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
We do provide the blocking peptide for product anti-FoxE3 antibody (A05314-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-01
