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Product Info Summary
| SKU: | A04495 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Forkhead box protein D3 FOXD3 Antibody
SKU/Catalog Number
A04495
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Forkhead box protein D3 FOXD3 Antibody (Catalog # A04495). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Forkhead box protein D3 FOXD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04495)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human FOXD3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04495 is reactive to FOXD3 in Human, Mouse
Reconstitution
Calculated molecular weight
47630 MW
Background of Foxd3
Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
Gregory S.G., Nature 441:315-321(2006).
Guo Y., Proc. Natl. Acad. Sci. U.S.A. 99:3663-3667(2002).
Ramsey H.C., Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04495 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HUVEC cells and 293 cells, using FOXD3 antibody A04495.
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using FOXD3 antibody A04495.
Protein Target Info & Infographic
Gene/Protein Information For FOXD3 (Source: Uniprot.org, NCBI)
Gene Name
FOXD3
Full Name
Forkhead box protein D3
Weight
47630 MW
Alternative Names
Forkhead box protein D3;HNF3/FH transcription factor genesis;FOXD3;HFH2; Foxd3|CWH3, Gen, Genesis, Hfh2|forkhead box D3|forkhead box protein D3|HFH-2|HNF-3/forkhead homolog 2|HNF3/FH transcription factor genesis|hepatocyte nuclear factor 3 forkhead homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXD3, check out the FOXD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Forkhead box protein D3 FOXD3 Antibody (A04495)
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No publications found for A04495
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Customer Q&As
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13 Customer Q&As for Anti-Forkhead box protein D3 FOXD3 Antibody
Question
I see that the anti-FOXD3 antibody A04495 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-20
Question
We need to test anti-FOXD3 antibody A04495 on human tibial nerve for research purposes, then I may be interested in using anti-FOXD3 antibody A04495 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
The products we sell, including anti-FOXD3 antibody A04495, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-20
Question
We are currently using anti-FOXD3 antibody A04495 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-FOXD3 antibody (A04495) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
Is a blocking peptide available for product anti-FOXD3 antibody (A04495)?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
We do provide the blocking peptide for product anti-FOXD3 antibody (A04495). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-05
Question
Can you help my question with product A04495, anti-FOXD3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04495 anti-FOXD3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-29
Question
Would anti-FOXD3 antibody A04495 work on canine WB with chronic myeloid leukemia cell?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
Our lab technicians have not validated anti-FOXD3 antibody A04495 on canine. You can run a BLAST between canine and the immunogen sequence of anti-FOXD3 antibody A04495 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine chronic myeloid leukemia cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-15
Question
Will anti-FOXD3 antibody A04495 work for IHC with tibial nerve?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
According to the expression profile of tibial nerve, FOXD3 is highly expressed in tibial nerve. So, it is likely that anti-FOXD3 antibody A04495 will work for IHC with tibial nerve.
Boster Scientific Support
Answered: 2019-08-09
Question
See below the WB image, lot number and protocol we used for tibial nerve using anti-FOXD3 antibody A04495. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-18
Question
Do you have a BSA free version of anti-FOXD3 antibody A04495 available?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FOXD3 antibody A04495 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-02
Question
I was wanting to use your anti-FOXD3 antibody for IHC for human tibial nerve on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tibial nerve identification?
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
It shows on the product datasheet, A04495 anti-FOXD3 antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human tibial nerve in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-28
Question
Is this A04495 anti-FOXD3 antibody reactive to the isotypes of FOXD3?
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
The immunogen of A04495 anti-FOXD3 antibody is Synthesized peptide derived from internal of human FOXD3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-24
Question
Will A04495 anti-FOXD3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
It shows on the product datasheet, A04495 anti-FOXD3 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibial nerve using anti-FOXD3 antibody A04495. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-15
