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Product Info Summary
| SKU: | M01032 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody
View all HNF-3 beta/FoxA2 Antibodies
SKU/Catalog Number
M01032
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody catalog # M01032. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01032)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAAH-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FOXA2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01032 is reactive to FOXA2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
48306 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01032 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of FOXA2 expression in HepG2 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For FOXA2 (Source: Uniprot.org, NCBI)
Gene Name
FOXA2
Full Name
Hepatocyte nuclear factor 3-beta
Weight
48306 MW
Alternative Names
Hepatocyte nuclear factor 3-beta;HNF-3-beta;HNF-3B;Forkhead box protein A2;Transcription factor 3B;TCF-3B;FOXA2;HNF3B, TCF3B; FOXA2 HNF-3-beta, HNF3B, TCF3B forkhead box A2 hepatocyte nuclear factor 3-beta|forkhead box protein A2|hepatic nuclear factor-3-beta|transcription factor 3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXA2, check out the FOXA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody (M01032)
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No publications found for M01032
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-FOXA2/Hnf3B Rabbit Monoclonal Antibody
Question
Is this M01032 anti-FOXA2/Hnf3B Rabbit Monoclonal antibody reactive to the isotypes of FOXA2?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
The immunogen of M01032 anti-FOXA2/Hnf3B Rabbit Monoclonal antibody is A synthesized peptide derived from human FOXA2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-25
Question
Will M01032 anti-FOXA2/Hnf3B Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
It shows on the product datasheet, M01032 anti-FOXA2/Hnf3B Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-14
Question
We are currently using anti-FOXA2/Hnf3B Rabbit Monoclonal antibody M01032 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
The anti-FOXA2/Hnf3B Rabbit Monoclonal antibody (M01032) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-FOXA2/Hnf3B Rabbit Monoclonal antibody M01032. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-18
Question
Our lab want to know about to test anti-FOXA2/Hnf3B Rabbit Monoclonal antibody M01032 on human liver for research purposes, then I may be interested in using anti-FOXA2/Hnf3B Rabbit Monoclonal antibody M01032 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Rodriguez
Verified customer
Asked: 2015-05-19
Answer
The products we sell, including anti-FOXA2/Hnf3B Rabbit Monoclonal antibody M01032, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-19
Question
Will anti-FOXA2/Hnf3B Rabbit Monoclonal antibody M01032 work on primate IF with liver?
T. Miller
Verified customer
Asked: 2015-03-17
Answer
Our lab technicians have not validated anti-FOXA2/Hnf3B Rabbit Monoclonal antibody M01032 on primate. You can run a BLAST between primate and the immunogen sequence of anti-FOXA2/Hnf3B Rabbit Monoclonal antibody M01032 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-17
