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Product Info Summary
| SKU: | A01569-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Fos B/FOSB Antibody
SKU/Catalog Number
A01569-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fos B/FOSB Antibody catalog # A01569-1. Tested in ELISA, IF, IHC, ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fos B/FOSB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01569-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOSB recombinant protein (Position: M1-S332).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01569-1 is reactive to FOSB in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
28681 MW
Background of FosB/G0S3
FOSB, FBJ murine osteosarcoma viral oncogene homolog B, is a protein that, in humans, is encoded by the FOSB gene. FOSB is a member of Fos gene family which consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. The FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. The FOSB gene is mapped to 19q13.32. Delta FOSB is a truncated splice variant of FOSB. Delta FosB has been implicated in the development of drug addiction and control of the reward system in the brain, and is linked to changes in a number of other gene products such as CREB and sirtuins. Delta FosB also regulates the commitment of mesenchymal precursor cells to the adipocyte or osteoblast lineage.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01569-1 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
IHC: human lung cancer tissue, human gallbladder adenocarcinoma tissue, human gallbladder adenocarcinoma tissue, human placenta tissue
ICC/IF: SiHa cell
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Fos B/FOSB using anti-Fos B/FOSB antibody (A01569-1).
Fos B/FOSB was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fos B/FOSB Antibody (A01569-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of Fos B/FOSB using anti-Fos B/FOSB antibody (A01569-1).
Fos B/FOSB was detected in a paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fos B/FOSB Antibody (A01569-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Fos B/FOSB using anti-Fos B/FOSB antibody (A01569-1).
Fos B/FOSB was detected in a paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fos B/FOSB Antibody (A01569-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Fos B/FOSB using anti-Fos B/FOSB antibody (A01569-1).
Fos B/FOSB was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fos B/FOSB Antibody (A01569-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of Fos B/FOSB using anti-Fos B/FOSB antibody (A01569-1).
Fos B/FOSB was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Fos B/FOSB Antibody (A01569-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For FOSB (Source: Uniprot.org, NCBI)
Gene Name
FOSB
Full Name
Protein fosB
Weight
28681 MW
Superfamily
bZIP family
Alternative Names
Vitamin K-dependent protein S; PROS1; PROS FOSB AP-1, G0S3, GOS3, GOSB FosB proto-oncogene, AP-1 transcription factor subunit protein fosB|FBJ murine osteosarcoma viral oncogene homolog B|FosB proto-oncogene, AP-1 trancription factor subunit|G0/G1 switch regulatory protein 3|activator protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOSB, check out the FOSB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOSB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fos B/FOSB Antibody (A01569-1)
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