Anti-FKBP12 FKBP1A Antibody

Product Name

Anti-FKBP12 FKBP1A Antibody

View all FKBP12 Antibodies

SKU/Catalog Number

A04492

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FKBP12 FKBP1A Antibody (Catalog # A04492). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

FKBP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-FKBP12 FKBP1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04492)

Host

Rabbit

Contents

FKBP12 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FKBP12 antibody was raised against an 18 amino acid peptide near the center of human FKBP12. The immunogen is located within the first 50 amino acids of FKBP12.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

FKBP12 antibody is human, mouse and rat reactive.

Reactive Species

A04492 is reactive to FKBP1A in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

11951 MW

Background of FKBP12

FKBP, or FK506 binding protein, is a family of proteins that have prolyl isomerase activity and are related to the cyclophilins in function (1). FKBP12, also called FKBP1, was originally identified as a target of FK506 and rapamycin activity and catalyzes the transition between cis and transproline residues critical for proper folding of proteins (2). FKBP12 is an abundant, evolutionarily conserved cytoplasmic protein that is an important regulator of diverse array of cellular processes including T cell activation, entry into the cell cycle and intracellular calcium release (3-5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04492 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FKBP12 antibody can be used for detection of FKBP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For FKBP1A (Source: Uniprot.org, NCBI)

Alternative Names

FKBP1, PKC12, PKCI2, FKBP12, PPIASE, FKBP-12, FKBP-1A, FKBP1, Peptidyl-prolyl cis-trans isomerase FKBP1A, 12 kDa FK506-binding protein, PPIase FKBP1A FKBP1A FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE FKBP prolyl isomerase 1A peptidyl-prolyl cis-trans isomerase FKBP1A|12 kDa FK506-binding protein|12 kDa FKBP|FK506 binding protein 1A, 12kDa|FK506 binding protein12|FK506-binding protein 1|FK506-binding protein 12|FK506-binding protein 1A|FK506-binding protein, T-cell, 12-kD|FKBP12-Exip3|PPIase FKBP1A|calstabin-1|immunophilin FKBP12|protein kinase C inhibitor 2|rotamase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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