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Product Info Summary
| SKU: | A05217-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-FKBP10 Antibody Picoband®
SKU/Catalog Number
A05217-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FKBP10 Antibody Picoband® catalog # A05217-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FKBP10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05217-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FKBP10 recombinant protein (Position: R29-E532).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05217-1 is reactive to FKBP10 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
39411 MW
Background of Fkbp10
FK506-binding protein 10 is a protein that in humans is encoded by the FKBP10 gene. The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05217-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A375 whole cell, human 293T whole cell, human Hela whole cell
ICC/IF: MCF-7 cell
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FKBP10 using anti-FKBP10 antibody (A05217-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP10 antigen affinity purified polyclonal antibody (Catalog # A05217-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP10 at approximately 72 kDa. The expected band size for FKBP10 is at 64 kDa.
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Figure 2. IF analysis of FKBP10 using anti-FKBP10 antibody (A05217-1).
FKBP10 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FKBP10 Antibody (A05217-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of A549 cells using anti-FKBP10 antibody (A05217-1).
Overlay histogram showing A549 cells stained with A05217-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FKBP10 Antibody (A05217-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For FKBP10 (Source: Uniprot.org, NCBI)
Gene Name
FKBP10
Full Name
Peptidyl-prolyl cis-trans isomerase FKBP10
Weight
39411 MW
Alternative Names
Histone deacetylase 10; HD10; HDAC10 Fkbp10|65kD, AI325255, FKBP-10, FKBP-65, FKBP65, Fkbp, Fkbp-, Fkbp-rs1, Fkbp1, Fkbp1-rs, Fkbp6, Fkbprp|FK506 binding protein 10|peptidyl-prolyl cis-trans isomerase FKBP10|65 kDa FK506-binding protein|65 kDa FKBP|FK506 binding protein 6 (65 kDa)|PPIase FKBP10|immunophilin FKBP65|rotamase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FKBP10, check out the FKBP10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FKBP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FKBP10 Antibody Picoband® (A05217-1)
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