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Product Info Summary
| SKU: | MA1116 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Mouse |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Fibronectin Fn1 Monoclonal Antibody
View all Fibronectin Antibodies
SKU/Catalog Number
MA1116
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fibronectin Fn1 Monoclonal Antibody catalog # MA1116. Tested in IHC, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibronectin Fn1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1116)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
FN-15
Isotype
Mouse IgG1
Immunogen
Fibronectin from human plasma.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1116 is reactive to FN1 in Chicken, Human, Mouse
Applications
MA1116 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
272511 MW
Background of Fibronectin
Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, chicken, mouse, By Heat
Immunocytochemistry , 1μg/ml, Human, chicken, mouse, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, chicken, mouse, -
Western blot, 1-2μg/ml, Human, chicken, mouse
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For FN1 (Source: Uniprot.org, NCBI)
Gene Name
FN1
Full Name
Fibronectin
Weight
272511 MW
Alternative Names
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF FN1 CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF fibronectin 1 fibronectin|cold-insoluble globulin|epididymis secretory sperm binding protein|migration-stimulating factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FN1, check out the FN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibronectin Fn1 Monoclonal Antibody (MA1116)
Hello CJ!
MA1116 has been cited in 26 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cdk5-mediated phosphorylation regulates phosphatidylinositol 4-phosphate 5-kinase type I γ 90 activity and cell invasion
Zhang X,Ke PX,Yuan X,Zhang GP,Chen WL,Zhang GS. Forskolin Protected against Streptozotocin-Induced Diabetic Cardiomyopathy via Inhibition of Oxidative Stress and Cardiac Fibrosis in Mice. Biomed Res Int.2021 Jan 29;2021:8881843.doi:10.1155/2021/8881843.PM
Species: Mouse
MA1116 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:NA
Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
MA1116 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:2000
Sang R,Liu Y,Kong L,Qian L,Liu C.Effect of Acellular Amnion With Increased TGF-β and bFGF Levels on the Biological Behavior of Tenocytes.Front Bioeng Biotechnol.2020 May 14;8:446.doi:10.3389/fbioe.2020.00446. PMID:32478059;PMCID:PMC7240037.
Species: Leghorn chicken
MA1116 usage in article: APP:IF, SAMPLE:ACELLULAR AMNIOTIC MEMBRANE, DILUTION:1:100
Glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in %u2026
Rosiglitazone prevents advanced glycation end products%u2013induced renal toxicity likely through suppression of plasminogen activator inhibitor-1
Contribution of fibronectin-binding protein to pathogenesis of Streptococcus equi ssp . zooepidemicus
Elongation Factor Thermo Unstable (EF-Tu) Moonlights as an Adhesin on the Surface of Mycoplasma hyopneumoniae by Binding to Fibronectin
Pathophysiology of chronic pancreatitis induced by dibutyltin dichloride joint ethanol in mice
Wu Y, Wang Y, Ji Y, Ou Y, Xia H, Zhang B, Zhao Y. Am J Transl Res. 2017 Dec 15;9(12):5708-5718. eCollection 2017. C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Fibronectin Fn1 Monoclonal Antibody
Question
We want to test anti-Fibronectin Monoclonal antibody MA1116 on chicken urine for research purposes, then I may be interested in using anti-Fibronectin Monoclonal antibody MA1116 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Evans
Verified customer
Asked: 2020-04-09
Answer
The products we sell, including anti-Fibronectin Monoclonal antibody MA1116, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-09
Question
I was wanting to use your anti-Fibronectin Monoclonal antibody for IHC for chicken urine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for chicken urine identification?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
You can see on the product datasheet, MA1116 anti-Fibronectin Monoclonal antibody has been validated for IHC, ICC, WB on chicken, human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in chicken urine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-13
Question
Does MA1116 anti-Fibronectin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
It shows on the product datasheet, MA1116 anti-Fibronectin Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-08
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urine using anti-Fibronectin Monoclonal antibody MA1116. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-27
Question
Is this MA1116 anti-Fibronectin Monoclonal antibody reactive to the isotypes of FN1?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
The immunogen of MA1116 anti-Fibronectin Monoclonal antibody is Fibronectin from human plasma. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-09
Question
See attached the WB image, lot number and protocol we used for urine using anti-Fibronectin Monoclonal antibody MA1116. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-14
Question
Is a blocking peptide available for product anti-Fibronectin Monoclonal antibody (MA1116)?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We do provide the blocking peptide for product anti-Fibronectin Monoclonal antibody (MA1116). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-18
Question
Is there a BSA free version of anti-Fibronectin Monoclonal antibody MA1116 available?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Fibronectin Monoclonal antibody MA1116 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-05
Question
Does anti-Fibronectin Monoclonal antibody MA1116 work on monkey WB with retinal pigment epithelium?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Our lab technicians have not validated anti-Fibronectin Monoclonal antibody MA1116 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Fibronectin Monoclonal antibody MA1116 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey retinal pigment epithelium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-11
Question
We have been able to see staining in chicken fibroblast. What should we do? Is anti-Fibronectin Monoclonal antibody supposed to stain fibroblast positively?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
According to literature fibroblast does express FN1. According to Uniprot.org, FN1 is expressed in tendon, retinal pigment epithelium, aortic endothelium, amygdala, cervix, colon endothelium, endometrial tumor
rc uterine endothelium, mammary cancer, fibroblast, plasma, peripheral blood t-cell umbilical vein endothelial cell, urine, cartilage, mammary carcinoma, cervix carcinoma, liver, among other tissues. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963
Boster Scientific Support
Answered: 2019-04-18
Question
My team were happy with the WB result of your anti-Fibronectin Monoclonal antibody. However we have seen positive staining in retinal pigment epithelium secreted using this antibody. Is that expected? Could you tell me where is FN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-18
Answer
From what I have seen in literature, retinal pigment epithelium does express FN1. Generally FN1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have FN1 expression, here are a few articles citing expression in various tissues:
Amygdala, Cervix, Colon endothelium, Endometrial tumor, and Uterine endothelium, Pubmed ID: 17974005
Cartilage, Pubmed ID: 12127832
Cervix carcinoma, Pubmed ID: 17081983
Fibroblast, Pubmed ID: 2992939
Liver, Pubmed ID: 18318008, 19159218, 24275569
Mammary cancer, Pubmed ID: 3770189
Mammary carcinoma, Pubmed ID: 6304699
Peripheral blood T-cell, and Umbilical vein endothelial cell, Pubmed ID: 8406884, 3024962
Plasma, Pubmed ID: 2012601, 6630202, 14760718, 16335952
Retinal pigment epithelium, Pubmed ID: 16106752
Urine, Pubmed ID: 17614963
Boster Scientific Support
Answered: 2018-07-18
Question
I have a question about product MA1116, anti-Fibronectin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1116 anti-Fibronectin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-11
Question
We purchased anti-Fibronectin Monoclonal antibody for WB on urine in the past. I am using human, and We are going to use the antibody for IHC next. We want examining urine as well as tendon in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
E. Patel
Verified customer
Asked: 2018-04-06
Answer
I have checked the website and datasheets of our anti-Fibronectin Monoclonal antibody and it appears that MA1116 has been validated on human in both WB and IHC. Thus MA1116 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-06
Question
I see that the anti-Fibronectin Monoclonal antibody MA1116 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-05
Question
Would anti-Fibronectin Monoclonal antibody MA1116 work for IHC with urine?
W. Gonzalez
Verified customer
Asked: 2014-11-24
Answer
According to the expression profile of urine, FN1 is highly expressed in urine. So, it is likely that anti-Fibronectin Monoclonal antibody MA1116 will work for IHC with urine.
Boster Scientific Support
Answered: 2014-11-24
Question
We are currently using anti-Fibronectin Monoclonal antibody MA1116 for chicken tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says chicken, human, mouse. Is it possible that the antibody can work on pig tissues as well?
C. Moore
Verified customer
Asked: 2014-09-11
Answer
The anti-Fibronectin Monoclonal antibody (MA1116) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-11
