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Product Info Summary
| SKU: | A03408-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-fibrillin 2/FBN2 Antibody Picoband®
View all Fibrillin 2 Antibodies
SKU/Catalog Number
A03408-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-fibrillin 2/FBN2 Antibody Picoband® catalog # A03408-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-fibrillin 2/FBN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03408-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human fibrillin 2/FBN2 recombinant protein (Position: N2740-Y2912).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03408-2 is reactive to FBN2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
315 kDa, 250 kDa
Calculated molecular weight
73109 MW
Background of Fibrillin 2
Fibrillin is a glycoprotein, which is essential for the formation of elastic fibers found in connective tissue. The protein encoded by this gene is a component of connective tissue microfibrils and may be involved in elastic fiber assembly. Mutations in this gene cause congenital contractural arachnodactyly.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03408-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human placenta tissue, human U20S whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Fibrillin 2/FBN2 using anti-Fibrillin 2/FBN2 antibody (A03408-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillin 2/FBN2 antigen affinity purified polyclonal antibody (Catalog # A03408-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillin 2/FBN2 at approximately 315,250 kDa. The expected band size for Fibrillin 2/FBN2 is at 315 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FBN2 (Source: Uniprot.org, NCBI)
Gene Name
FBN2
Full Name
Fibrillin-2
Weight
73109 MW
Superfamily
fibrillin family
Alternative Names
Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K (+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1 FBN2 CCA, DA9, EOMD fibrillin 2 fibrillin-2|fibrillin 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FBN2, check out the FBN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FBN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-fibrillin 2/FBN2 Antibody Picoband® (A03408-2)
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