Product Info Summary
SKU: | M01200 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-FHIT Antibody Picoband™ (monoclonal, 26H7)
SKU/Catalog Number
M01200
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FHIT Antibody Picoband™ (monoclonal, 26H7) catalog # M01200. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FHIT Antibody Picoband™ (monoclonal, 26H7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01200)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
26H7
Isotype
Mouse IgG1
Immunogen
E.coli-derived human FHIT recombinant protein (Position: M1-Q147). Human FHIT shares 90% and 87% amino acid (aa) sequence identity with mouse and rat FHIT, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01200 is reactive to FHIT in Human
Applications
M01200 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
17 kDa
Calculated molecular weight
Background of FHIT
Bis (5'-adenosyl)-triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1,P3-bis (5'-adenosyl)-triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
![M01200 FHIT primary antibodies WB testing 1_1 M01200 FHIT primary antibodies WB testing 1_1](https://www.bosterbio.com/media/catalog/product/M/0/M01200-FHIT-primary-antibodies-WB-testing-1_1.jpg)
Click image to see more details
Figure 1. Western blot analysis of FHIT using anti-FHIT antibody (M01200).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human COLO-320 whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human SGC-7901 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FHIT antigen affinity purified monoclonal antibody (Catalog # M01200) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
![m01200 fhit primary antibodies if testing 2 m01200 fhit primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m01200-fhit-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of FHIT using anti-FHIT antibody (M01200).
FHIT was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-FHIT Antibody (M01200) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![m01200 fhit primary antibodies fcm testing 3 m01200 fhit primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m01200-fhit-primary-antibodies-fcm-testing-3.jpg)
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Figure 3. Flow Cytometry analysis of 293T cells using anti-FHIT antibody (M01200).
Overlay histogram showing 293T cells stained with M01200 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-FHIT Antibody (M01200,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For FHIT (Source: Uniprot.org, NCBI)
Gene Name
FHIT
Full Name
Bis(5'-adenosyl)-triphosphatase
Weight
Alternative Names
AP3A hydrolase; AP3AaseFRA3Bbis(5'-adenosyl)-triphosphatase; Diadenosine 5'-5'''-P1; dinucleosidetriphosphatase; EC 3.6.1.29; fragile histidine triad gene; Fragile histidine triad protein; P3-triphosphate hydrolase; tumor suppressor protein FHIT AP3Aase, FRA3B fragile histidine triad diadenosine triphosphatase bis(5-adenosyl)-triphosphatase|AP3A hydrolase|diadenosine 5,5-P1,P3-triphosphate hydrolase|dinucleosidetriphosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FHIT, check out the FHIT Infographic
![FHIT infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FHIT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FHIT Antibody Picoband™ (monoclonal, 26H7) (M01200)
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