Anti-FGFR4 Rabbit Monoclonal Antibody

Product Name

Anti-FGFR4 Rabbit Monoclonal Antibody

View all FGFR4 Antibodies

SKU/Catalog Number

M00769

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-FGFR4 Rabbit Monoclonal Antibody catalog # M00769. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FGFR4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00769)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABHA-6

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human FGFR4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00769 is reactive to FGFR4 in Human, Mouse, Rat

Applications

M00769 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

88 kDa

Calculated molecular weight

87954 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FGFR4 using anti-FGFR4 antibody (M00769).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 antigen affinity purified monoclonal antibody (Catalog # M00769) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR4 at approximately 88 kDa. The expected band size for FGFR4 is at 88 kDa.

Gene/Protein Information For FGFR4 (Source: Uniprot.org, NCBI)

Alternative Names

CD334 antigen; CD334; EC 2.7.10; EC 2.7.10.1; FGF R4; FGFR4; FGFR-4; fibroblast growth factor receptor 4; JTK2hydroxyaryl-protein kinase; MGC20292; protein-tyrosine kinase; TKF; tyrosine kinase related to fibroblast growth factor receptor; tyrosylprotein kinase FGFR4 CD334, JTK2, TKF fibroblast growth factor receptor 4 fibroblast growth factor receptor 4|hydroxyaryl-protein kinase|protein-tyrosine kinase|tyrosine kinase related to fibroblast growth factor receptor|tyrosylprotein kinase

For more info on FGFR4, check out the FGFR4 Infographic

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In this infographic, you will see the following information for FGFR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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