Click image to see more details
Product Info Summary
| SKU: | M00769 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-FGFR4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00769
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FGFR4 Rabbit Monoclonal Antibody catalog # M00769. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGFR4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00769)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABHA-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FGFR4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00769 is reactive to FGFR4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
88 kDa
Calculated molecular weight
87954 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00769 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: human MCF-7 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FGFR4 using anti-FGFR4 antibody (M00769).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 antigen affinity purified monoclonal antibody (Catalog # M00769) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR4 at approximately 88 kDa. The expected band size for FGFR4 is at 88 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FGFR4 (Source: Uniprot.org, NCBI)
Gene Name
FGFR4
Full Name
Fibroblast growth factor receptor 4
Weight
87954 MW
Superfamily
protein kinase superfamily
Alternative Names
Fibroblast growth factor receptor 4;FGFR-4;2.7.10.1;CD334;FGFR4;JTK2, TKF; FGFR4 CD334, JTK2, TKF fibroblast growth factor receptor 4 fibroblast growth factor receptor 4|hydroxyaryl-protein kinase|protein-tyrosine kinase|tyrosine kinase related to fibroblast growth factor receptor|tyrosylprotein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGFR4, check out the FGFR4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGFR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGFR4 Rabbit Monoclonal Antibody (M00769)
Hello CJ!
No publications found for M00769
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-FGFR4 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-FGFR4 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-FGFR4 Rabbit Monoclonal Antibody
Question
Would anti-FGFR4 Rabbit Monoclonal antibody M00769 work for WB with blood?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
According to the expression profile of blood, FGFR4 is highly expressed in blood. So, it is likely that anti-FGFR4 Rabbit Monoclonal antibody M00769 will work for WB with blood.
Boster Scientific Support
Answered: 2020-03-02
Question
I was wanting to use your anti-FGFR4 Rabbit Monoclonal antibody for WB for mouse blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse blood identification?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
As indicated on the product datasheet, M00769 anti-FGFR4 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-13
Question
Is there a BSA free version of anti-FGFR4 Rabbit Monoclonal antibody M00769 available?
R. Bhatt
Verified customer
Asked: 2019-11-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGFR4 Rabbit Monoclonal antibody M00769 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-20
Question
My question regarding product M00769, anti-FGFR4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00769 anti-FGFR4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-28
Question
Is a blocking peptide available for product anti-FGFR4 Rabbit Monoclonal antibody (M00769)?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
We do provide the blocking peptide for product anti-FGFR4 Rabbit Monoclonal antibody (M00769). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-14
Question
Would M00769 anti-FGFR4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
You can see on the product datasheet, M00769 anti-FGFR4 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-08
