Product Info Summary
SKU: | A00802-Biotin |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA |
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Product info
Product Name
Anti-FGF21 Biotinylated Antibody
SKU/Catalog Number
A00802-Biotin
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGF21 Biotinylated Antibody catalog # A00802-Biotin. Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF21 Biotinylated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00802-Biotin)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FGF21 recombinant protein (Position: H29-S209).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00802-Biotin is reactive to FGF21 in Human
Applications
A00802-Biotin is guaranteed for ELISA Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.3kDa
Background of FGF-21
FGF21 (Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor (FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Detection (Matched Antibody Pair), 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For FGF21 (Source: Uniprot.org, NCBI)
Gene Name
FGF21
Full Name
Fibroblast growth factor 21
Weight
22.3kDa
Superfamily
heparin-binding growth factors family
Alternative Names
FGF21; FGF-21; fibroblast growth factor 21 FGF21 fibroblast growth factor 21 fibroblast growth factor 21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF21, check out the FGF21 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF21 Biotinylated Antibody (A00802-Biotin)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-FGF21 Biotinylated Antibody
Question
We have seen staining in human right lobe of liver. Any tips? Is anti-FGF21 Biotinylated antibody supposed to stain right lobe of liver positively?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
Based on literature right lobe of liver does express FGF21. Based on Uniprot.org, FGF21 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-03-15
Question
We were well pleased with the WB result of your anti-FGF21 Biotinylated antibody. However we have observed positive staining in right lobe of liver secreted. using this antibody. Is that expected? Could you tell me where is FGF21 supposed to be expressed?
Z. Wu
Verified customer
Asked: 2019-01-30
Answer
From literature, right lobe of liver does express FGF21. Generally FGF21 expresses in secreted. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-30
Question
We are currently using anti-FGF21 Biotinylated antibody A00802-Biotin for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
A. Zhao
Verified customer
Asked: 2017-05-16
Answer
The anti-FGF21 Biotinylated antibody (A00802-Biotin) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-16