Product Info Summary
SKU: | PA1421 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Alpha-2-HS-glycoprotein AHSG Antibody
SKU/Catalog Number
PA1421
BA4757-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Alpha-2-HS-glycoprotein AHSG Antibody catalog # PA1421. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha-2-HS-glycoprotein AHSG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1421)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Fetuin A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1421 is reactive to AHSG in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55-60 kDa
Calculated molecular weight
39325 MW
Background of AHSG
Alpha-2-HS-glycoprotein (AHSG) also known as fetuin-A is a protein that in humans is encoded by the AHSG gene. Fetuin-A belongs to the fetuin class of plasma binding proteins and is more abundant in fetal than adult blood. Its gene is mapped to 3q27. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1421 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human placenta tissue, human HCCT tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Fetuin A using anti-Fetuin A antibody (PA1421).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HCCT tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fetuin A antigen affinity purified polyclonal antibody (Catalog # PA1421) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fetuin A at approximately 55-60 kDa. The expected band size for Fetuin A is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For AHSG (Source: Uniprot.org, NCBI)
Gene Name
AHSG
Full Name
Alpha-2-HS-glycoprotein
Weight
39325 MW
Superfamily
fetuin family
Alternative Names
Alpha-2-HS-glycoprotein;Alpha-2-Z-globulin;Ba-alpha-2-glycoprotein;Fetuin-A;Alpha-2-HS-glycoprotein chain A;Alpha-2-HS-glycoprotein chain B;AHSG;FETUA;PRO2743; AHSG A2HS, AHS, APMR1, FETUA, HSGA alpha 2-HS glycoprotein alpha-2-HS-glycoprotein|alpha-2-Z-globulin|ba-alpha-2-glycoprotein|fetuin-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AHSG, check out the AHSG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AHSG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha-2-HS-glycoprotein AHSG Antibody (PA1421)
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No publications found for PA1421
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Customer Q&As
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3 Customer Q&As for Anti-Alpha-2-HS-glycoprotein AHSG Antibody
Question
I would like to test anti-AHSG antibody PA1421 on human platelet for research purposes, then I may be interested in using anti-AHSG antibody PA1421 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
The products we sell, including anti-AHSG antibody PA1421, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-10
Question
I was wanting to use your anti-AHSG antibody for WB for human platelet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
It shows on the product datasheet, PA1421 anti-AHSG antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-31
Question
Will PA1421 anti-AHSG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
You can see on the product datasheet, PA1421 anti-AHSG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-07