Anti-Fbp5B FBXO43 Antibody

Product Name

Anti-Fbp5B FBXO43 Antibody

View all F-box protein 43 Antibodies

SKU/Catalog Number

A12000

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Fbp5B FBXO43 Antibody (Catalog # A12000). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat, Chimpanzee.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Fbp5B FBXO43 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12000)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein. Human Fbp5B-2, 466 aa, predicted MW 51.3 kDa

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12000 is reactive to Fbxo43 in Human, Mouse, Rat, Chimpanzee

Applications

A12000 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

52329 MW

Background of F-box protein 43

Fbp5B (also called F-Box protein 43 or Fbx043) is a relatively new member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IP: 1:100
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of FBXO43, EMI2 using anti-FBXO43, EMI2 antibody (A12000).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO43, EMI2 antigen affinity purified polyclonal antibody (Catalog # A12000) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for FBXO43, EMI2.

Gene/Protein Information For Fbxo43 (Source: Uniprot.org, NCBI)

Alternative Names

early mitotic inhibitor 2; EMI2Fbx43; Endogenous meiotic inhibitor 2; ERP1; F-box only protein 43; F-box protein 43; FBX43 Fbxo43|4930533G20Rik, Emi, Emi2|F-box protein 43|F-box only protein 43|XErp1 homolog|early mitotic inhibitor 2|endogenous meiotic inhibitor 2

For more info on Fbxo43, check out the Fbxo43 Infographic

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In this infographic, you will see the following information for Fbxo43: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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