Click image to see more details
Product Info Summary
| SKU: | PA1853 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband®
View all FABP5/E-FABP Antibodies
SKU/Catalog Number
PA1853
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fatty Acid Binding Protein 5/FABP5 Antibody catalog # PA1853. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1853)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Fatty Acid Binding Protein 5, different from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1853 is reactive to Fabp5 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
15137 MW
Background of FABP5/E-FABP
FABP5 (fatty acid binding protein 5 (psoriasis-associated)) is a protein that in humans is encoded by the FABP5 gene, also known as PAFABP, EFABP, E-FABP, KFABP, PA-FABP, Epidermal-type fatty acid-binding protein, Fatty acid-binding protein 5, Psoriasis-associated fatty acid-binding protein homolog. It is a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. The PAFABP cDNA encodes a 135-amino acid protein with molecular weight 15,164. This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. It is though that FABPs roles include fatty acid uptake, transport, and metabolism. Kaczocha et al. (2009) identified FABP5 and FABP7 as cytosolic proteins that transport AEA from the plasma membrane to subcellular fatty acid amide hydrolase, where it is hydrolyzed and inactivated. FABP3 did not show this specific transport function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1853 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat
Positive Control
WB: rat lung tissue, rat intestine tissuemouse lung tissue
IHC: mouse intestine tissue, rat intestine tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FABP5 using anti-FABP5 antibody (PA1853).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat intestine tissue lysates.
Lane 3: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP5 antigen affinity purified polyclonal antibody (Catalog # PA1853) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP5 at approximately 15 kDa. The expected band size for FABP5 is at 15 kDa.
Click image to see more details
Figure 2. IHC analysis of FABP5 using anti-FABP5 antibody (PA1853).
FABP5 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FABP5 Antibody (PA1853) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of FABP5 using anti-FABP5 antibody (PA1853).
FABP5 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FABP5 Antibody (PA1853) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Fabp5 (Source: Uniprot.org, NCBI)
Gene Name
Fabp5
Full Name
Fatty acid-binding protein 5
Weight
15137 MW
Superfamily
calycin superfamily
Alternative Names
Fatty acid-binding protein, epidermal;Epidermal-type fatty acid-binding protein;E-FABP;Fatty acid-binding protein 5;Keratinocyte lipid-binding protein;Psoriasis-associated fatty acid-binding protein homolog;PA-FABP;Fabp5;Fabpe, Klbp, Mal1; FABP5 E-FABP, EFABP, KFABP, PA-FABP, PAFABP fatty acid binding protein 5 fatty acid-binding protein 5|epidermal-type fatty acid-binding protein|fatty acid binding protein 5 (psoriasis-associated)|psoriasis-associated fatty acid-binding protein homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Fabp5, check out the Fabp5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fabp5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband® (PA1853)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Fatty Acid Binding Protein 5/FABP5 antibody (PA1853)?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do provide the blocking peptide for product anti-Fatty Acid Binding Protein 5/FABP5 antibody (PA1853). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-05
Question
We are currently using anti-Fatty Acid Binding Protein 5/FABP5 antibody PA1853 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
The anti-Fatty Acid Binding Protein 5/FABP5 antibody (PA1853) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-04
Question
We are interested in to test anti-Fatty Acid Binding Protein 5/FABP5 antibody PA1853 on mouse keratinocyte for research purposes, then I may be interested in using anti-Fatty Acid Binding Protein 5/FABP5 antibody PA1853 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
The products we sell, including anti-Fatty Acid Binding Protein 5/FABP5 antibody PA1853, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for keratinocyte using anti-Fatty Acid Binding Protein 5/FABP5 antibody PA1853. Let me know if you need anything else.
M. Williams
Verified customer
Asked: 2017-06-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-29
Question
I have a question about product PA1853, anti-Fatty Acid Binding Protein 5/FABP5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Roberts
Verified customer
Asked: 2013-07-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1853 anti-Fatty Acid Binding Protein 5/FABP5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-31
