Product Info Summary
SKU: | M01411-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IF, IHC-P |
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Product info
Product Name
Anti-Collagen IV COL4A1 Monoclonal Antibody
View all Collagen IV alpha 1 Antibodies
SKU/Catalog Number
M01411-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Collagen IV COL4A1 Monoclonal Antibody catalog # M01411-1. Tested in IF, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen IV COL4A1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01411-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Q32
Isotype
IgG1
Immunogen
synthetic peptide derived from Collagen IV.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01411-1 is reactive to COL4A1 in Human, Mouse, Rat
Applications
M01411-1 is guaranteed for IF, IHC-P Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
160611 MW
Background of Collagen IV alpha 1
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF 1:50-200
IHC-p 1:50-300
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Collagen IV Mouse monoclonal antibody (8E5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, Collagen IV Mouse monoclonal antibody (8E5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunofluorescence analysis of mouse-spleen tissue. 1, Collagen IV Mouse monoclonal antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Click image to see more details
Immunofluorescence analysis of rat-lung tissue. 1, Collagen IV Mouse monoclonal antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Click image to see more details
Immunofluorescence analysis of human-liver tissue. 1, Collagen IV Mouse monoclonal antibody (8E5) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Protein Target Info & Infographic
Gene/Protein Information For COL4A1 (Source: Uniprot.org, NCBI)
Gene Name
COL4A1
Full Name
Collagen alpha-1(IV) chain
Weight
160611 MW
Superfamily
type IV collagen family
Alternative Names
alpha-1 chain; Arresten; COL4A1 NC1 domain; COL4A1; collagen alpha-1(IV) chain; Collagen IV alpha 1; collagen IV, alpha-1 polypeptide; collagen, type IV, alpha 1; Collagen-4; EC 3.4.23; EC 6.3.1.2 COL4A1 BSVD, BSVD1s, PADMAL, RATOR, COL4A1 collagen type IV alpha 1 chain collagen alpha-1(IV) chain|COL4A1 NC1 domain|arresten|collagen IV, alpha-1 polypeptide|collagen of basement membrane, alpha-1 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL4A1, check out the COL4A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen IV COL4A1 Monoclonal Antibody (M01411-1)
Hello CJ!
M01411-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang, Y., Deng, R., Chen, Z., Yao, L., Yang, X., & Xiang, D. (2021). Piperazine ferulate attenuates high glucose‑induced mesangial cell injury via the regulation of p66Shc. Molecular Medicine Reports, 23, 374. https://doi.org/10.3892/mmr.2021.12013
Species: Mouse
M01411-1 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
Species: Human,Rat
M01411-1 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Zhou S,Ma Y,Zhao D,Mi Y,Zhang C. Transcriptome profiling analysis of underlying regulation of growing follicle development in the chicken. Poult Sci.2020 Jun;99(6):2861-2872. doi:10.1016/j.psj.2019.12.067. Epub 2020 Mar 19.PMID:32475419;PMCID:PMC7597661.
Species: Hy-Line white laying hens
M01411-1 usage in article: APP:IHC, SAMPLE:OVARIES, DILUTION:1:100
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