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Product Info Summary
| SKU: | PA2061 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Factor VIII/F8 Antibody Picoband®
SKU/Catalog Number
PA2061
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Factor VIII/F8 Antibody catalog # PA2061. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor VIII/F8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2061)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Factor VIII.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2061 is reactive to F8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
92 kDa
Calculated molecular weight
267009 MW
Background of F8
Coagulation Factor VIII (FVIII) is an essential blood-clotting protein, also known as anti-hemophilic factor (AHF). By in situ hybridization, Tantravahi et al. (1986) concluded that the F8 gene is located in the proximal part of chromosome Xq28 with probes DX13 and St14 distally located. The F8 gene encodes coagulation factor VIII, a large plasma glycoprotein that functions in the blood coagulation cascade as a cofactor for the factor IXa-dependent activation of factor X (F10). Factor VIII is activated proteolytically by a variety of coagulation enzymes, including thrombin (F2). Factor VIII is tightly associated in the blood with von Willebrand factor (VWF), which serves as a protective carrier protein for factor VIII (Toole et al., 1984; Hoyer, 1994).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2061 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat kidney tissue, rat lung tissue, rat heart tissue, mouse kidney tissue, mouse lung tissue, human Hela whole cell, human HepG2 whole cell,
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Factor VIII using anti-Factor VIII antibody (PA2061).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human HepG2 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor VIII antigen affinity purified polyclonal antibody (Catalog # PA2061) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor VIII at approximately 92KD. The expected band size for Factor VIII is at 92KD.
Protein Target Info & Infographic
Gene/Protein Information For F8 (Source: Uniprot.org, NCBI)
Gene Name
F8
Full Name
Coagulation factor VIII
Weight
267009 MW
Superfamily
multicopper oxidase family
Alternative Names
Coagulation factor VIII;Antihemophilic factor;AHF;Procoagulant component;Factor VIIIa heavy chain, 200 kDa isoform;Factor VIIIa heavy chain, 92 kDa isoform;Factor VIII B chain;Factor VIIIa light chain;F8;F8C; F8 AHF, DXS1253EB, F8C, FVIII, HEMA, F8 coagulation factor VIII coagulation factor VIII|antihemophilic factor|coagulation factor VIII A1 domain|coagulation factor VIII C2 domain|coagulation factor VIII, procoagulant component|coagulation factor VIIIc|factor VIII F8B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F8, check out the F8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Factor VIII/F8 Antibody Picoband® (PA2061)
Hello CJ!
PA2061 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Paeonol promotes microRNA-126 expression to inhibit monocyte adhesion to ox-LDL-injured vascular endothelial cells and block the activation of the PI3K %u2026
RNA-seq analysis provide new insights into mapk signaling of apolipoproteinciii-induced inflammation in porcine vascular endothelial cells
Zhang X, Shen Mr, Xu Zd, Hu Z, Chen C, Chi Yl, Kong Zd, Li Zf, Li Xt, Guo Sl, Xiong Sh, Zhang Cs. Exp Biol Med (Maywood). 2014 May;239(5):628-37. Doi: 10.1177/1535370214525323. Epub 2014 Mar 27. Cardiomyocyte Differentiation Induced In Cardiac Pro...
Li S, Qu H, Hao J, Sun J, Guo H, Guo C, Sun B, Tu C. Biochim Biophys Acta. 2010 Sep;1804(9):1882-8. Doi: 10.1016/J.Bbapap.2010.05.011. Epub 2010 Jun 1. Proteomic Analysis Of Primary Porcine Endothelial Cells After Infection By Classical Swine Feve...
Li Y, Cao Y, Zeng Z, Liang M, Xue Y, Xi C, Zhou M, Jiang W. Sci Rep. 2015 Feb 3;5:8209. Doi: 10.1038/Srep08209. Angiotensin-Converting Enzyme 2/Angiotensin-(1-7)/Mas Axis Prevents Lipopolysaccharide-Induced Apoptosis Of Pulmonary Microvascular End...
Zhang E, Shen F,. J Biomed Mater Res A. 2015 Aug;103(8):2758-69. Doi: 10.1002/Jbm.A.35410. Epub 2015 Feb 24. A Ferulic Acid (Fa)-Eluting System For Biodegradable Magnesium Stent: Cells Response Of Huvecs.
You Qh, Sun Gy, Wang N, Shen Jl, Wang Y. J Surg Res. 2010 Jul;162(1):110-21. Doi: 10.1016/J.Jss.2009.01.019. Epub 2009 Feb 8. Interleukin-17F-Induced Pulmonary Microvascular Endothelial Monolayer Hyperpermeability Via The Protein Kinase C Pathway.
Feng D, Peng C, Li C, Zhou Y, Li M, Ling B, Wei H, Tian Z. Oncol Rep. 2009 Nov;22(5):1129-34. Identification And Characterization Of Cancer Stem-Like Cells From Primary Carcinoma Of The Cervix Uteri.
Wang J, Chen Y, Lin C, Jia J, Tian L, Yang K, Zhao L, Lai N, Jiang Q, Sun Y, Zhong N, Ran P, Lu W. Am J Physiol Cell Physiol. 2014 Feb 15;306(4):C364-73. Doi: 10.1152/Ajpcell.00048.2013. Epub 2013 Dec 11. Effects Of Chronic Exposure To Cigarette S...
Liu X, Zhang G, Hou C, Wang H, Yang Y, Guan G, Dong W, Gao H, Feng Q. Biomed Res Int. 2013;2013:854917. Doi: 10.1155/2013/854917. Epub 2013 Dec 4. Vascularized Bone Tissue Formation Induced By Fiber-Reinforced Scaffolds Cultured With Osteoblasts A...
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Customer Q&As
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1 Customer Q&As for Anti-Factor VIII/F8 Antibody Picoband®
Question
We are currently using anti-Factor VIII/F8 antibody PA2061 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
The anti-Factor VIII/F8 antibody (PA2061) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-30
