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Product Info Summary
| SKU: | A00537 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Factor IX F9 Antibody
SKU/Catalog Number
A00537
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Factor IX F9 Antibody catalog # A00537. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor IX F9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00537)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Factor IX at AA range: 412-461
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00537 is reactive to F9 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
51778 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00537 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of 3T3 HepG2 cells using Factor IX Polyclonal antibody diluted at 1:800.
Protein Target Info & Infographic
Gene/Protein Information For F9 (Source: Uniprot.org, NCBI)
Gene Name
F9
Full Name
Coagulation factor IX
Weight
51778 MW
Superfamily
peptidase S1 family
Alternative Names
Coagulation factor IX ;3.4.21.22 ;Christmas factor;Plasma thromboplastin component;PTC;Coagulation factor IXa light chain;Coagulation factor IXa heavy chain;F9; F9 F9 p22, FIX, HEMB, P19, PTC, THPH8 coagulation factor IX coagulation factor IX|Christmas factor|factor 9|factor IX F9|plasma thromboplastic component|plasma thromboplastin component
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F9, check out the F9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Factor IX F9 Antibody (A00537)
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No publications found for A00537
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Factor IX F9 Antibody
Question
Does anti-Factor IX antibody A00537 work on zebrafish WB with liver?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Our lab technicians have not tested anti-Factor IX antibody A00537 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Factor IX antibody A00537 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
Is a blocking peptide available for product anti-Factor IX antibody (A00537)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We do provide the blocking peptide for product anti-Factor IX antibody (A00537). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-04
Question
Does A00537 anti-Factor IX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
As indicated on the product datasheet, A00537 anti-Factor IX antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-18
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Factor IX antibody A00537. Please let me know if you require anything else.
D. Krishna
Verified customer
Asked: 2015-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-06
Question
We are currently using anti-Factor IX antibody A00537 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
N. Krishna
Verified customer
Asked: 2013-02-22
Answer
The anti-Factor IX antibody (A00537) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-22
