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Product Info Summary
| SKU: | A00361-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-ETV6 Antibody (N-term)
SKU/Catalog Number
A00361-2
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-ETV6 Antibody (N-term) (Catalog # A00361-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ETV6 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00361-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ETV6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-111 amino acids from the N-terminal region of human ETV6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00361-2 is reactive to ETV6 in Human
Reconstitution
Calculated molecular weight
53000 Da
Background of ETV6/Tel
ETV6 is an ETS family transcription factor. This protein contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00361-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-ETV6 Antibody (N-term) at 1:2000 dilution
Lane 1: U266B1 whole cell lysate
Lane 2: U-2OS whole cell lysate
Lane 3: Raji whole cell lysate
Lane 4: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human testis tissue reacted with ETV6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ETV6 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For ETV6 (Source: Uniprot.org, NCBI)
Gene Name
ETV6
Full Name
Transcription factor ETV6
Weight
53000 Da
Superfamily
ETS family
Alternative Names
Transcription factor ETV6, ETS translocation variant 6, ETS-related protein Tel1, Tel, ETV6, TEL, TEL1 ETV6 TEL, TEL/ABL, THC5 ETS variant transcription factor 6 transcription factor ETV6|ETS translocation variant 6|ETS variant 6|ETS-related protein Tel1|TEL1 oncogene|ets variant gene 6 (TEL oncogene)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ETV6, check out the ETV6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ETV6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ETV6 Antibody (N-term) (A00361-2)
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No publications found for A00361-2
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