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Product Info Summary
| SKU: | M00786-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody
View all ER beta/NR3A2 Antibodies
SKU/Catalog Number
M00786-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody catalog # M00786-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00786-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IDI-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Estrogen Receptor beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00786-1 is reactive to ESR2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
59 kDa
Calculated molecular weight
59216 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00786-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human cervix cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (M00786-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified monoclonal antibody (Catalog # M00786-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59 kDa. The expected band size for ESR2 is at 59 kDa.
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Immunohistochemical analysis of paraffin-embedded human cervix cancer, using Estrogen Receptor beta Antibody.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For ESR2 (Source: Uniprot.org, NCBI)
Gene Name
ESR2
Full Name
Estrogen receptor beta
Weight
59216 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Estrogen receptor beta;ER-beta;Nuclear receptor subfamily 3 group A member 2;ESR2;ESTRB, NR3A2; ESR2 ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8 estrogen receptor 2 estrogen receptor beta|estrogen receptor beta 4|estrogen receptor beta splice variant, ERbeta2delta7|estrogen receptor beta splice variant, ERbeta4delta7|estrogen receptor beta splice variant, ERbeta6|estrogen receptor beta splice variant, ERbeta6delta7|estrogen receptor beta splice variant, ERbeta7|estrogen receptor beta splice variant, ERbeta7delta7|estrogen receptor beta splice variant, ERbetaEx. 4L|estrogen receptor beta splice variant, ERbetaEx. 6L|nuclear receptor subfamily 3 group A member 2|oestrogen receptor beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ESR2, check out the ESR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ESR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody (M00786-1)
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