Anti-Estrogen Receptor beta/ESR2 Antibody Picoband®

Product Name

Anti-Estrogen Receptor beta/ESR2 Antibody Picoband®

View all ER beta/NR3A2 Antibodies

SKU/Catalog Number

PA1126

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Estrogen Receptor beta/ESR2 Antibody catalog # PA1126. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Estrogen Receptor beta/ESR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1126)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Estrogen Receptor beta, different from the related rat and mouse sequences by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1126 is reactive to ESR2 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

65 kDa

Calculated molecular weight

59216 MW

Background of ER beta/NR3A2

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1126 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: MCF-7 Cell

Validation Images & Assay Conditions

Gene/Protein Information For ESR2 (Source: Uniprot.org, NCBI)

Alternative Names

Estrogen receptor beta;ER-beta;Nuclear receptor subfamily 3 group A member 2;ESR2;ESTRB, NR3A2; ESR2 ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8 estrogen receptor 2 estrogen receptor beta|estrogen receptor beta 4|estrogen receptor beta splice variant, ERbeta2delta7|estrogen receptor beta splice variant, ERbeta4delta7|estrogen receptor beta splice variant, ERbeta6|estrogen receptor beta splice variant, ERbeta6delta7|estrogen receptor beta splice variant, ERbeta7|estrogen receptor beta splice variant, ERbeta7delta7|estrogen receptor beta splice variant, ERbetaEx. 4L|estrogen receptor beta splice variant, ERbetaEx. 6L|nuclear receptor subfamily 3 group A member 2|oestrogen receptor beta

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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