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Product Info Summary
| SKU: | M01464-1 |
|---|---|
| Size: | 200ug |
| Reactive Species: | Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat |
| Host: | Mouse |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ERp57 (GRP58) Monoclonal Antibody
View all ERp57/PDIA3 Antibodies
SKU/Catalog Number
M01464-1
Size
200ug
Form
liquid
Description
Boster Bio Anti-ERp57 (GRP58) Monoclonal Antibody catalog # M01464-1. Tested in IP, IF, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERp57 (GRP58) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01464-1)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
Map.ERp57
Isotype
IgG1
Immunogen
Human recombinant ERp57 (Grp58)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~57kDa.
Reactive Species
M01464-1 is reactive to ERp57 in Bovine, Dog, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
20697 MW
Background of ERp57/PDIA3
ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01464-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (M01464-1) . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (M01464-1) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and perinuclear staining.
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Figure 2. IHC analysis of PDIA3 using anti-PDIA3 antibody (M01464-1).
PDIA3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDIA3 Antibody (M01464-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 3. Western blot analysis of PDIA3 using anti-PDIA3 antibody (M01464-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIA3 antigen affinity purified polyclonal antibody (Catalog # M01464-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for PDIA3.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 (Grp58) Monoclonal Antibody, Clone Map.ERP57 (M01464-1) . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Erp57 (Grp58) Monoclonal Antibody (M01464-1) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erp57 (Grp58) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Protein Target Info & Infographic
Gene/Protein Information For ERp57 (Source: Uniprot.org, NCBI)
Gene Name
ERp57
Full Name
Protein disulfide-isomerase A3
Weight
20697 MW
Superfamily
protein disulfide isomerase family
Alternative Names
ERp60, ERp61, Grp57, Grp58, P58, PDIA3, PI PLC, 58 kDa glucose regulated protein, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER 60, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, ER p57, ER protein 57, ER protein 60, ERp 57, ERp57, Glucose Regulated Protein 58 Kd, GRP 57, GRP 58, GRP57, HsT17083, p58, PDIA 3, PDIA3, PDIA3_HUMAN, Phospholipase C alpha, PI PLC, Protein disulfide isomerase A3, Protein disulfide isomerase family A member 3, Protein disulfide-isomerase A3 PDIA3 ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC protein disulfide isomerase family A member 3 protein disulfide-isomerase A3|58 kDa glucose-regulated protein|58 kDa microsomal protein|ER protein 57|ER protein 60|disulfide isomerase ER-60|endoplasmic reticulum P58|endoplasmic reticulum resident protein 57|endoplasmic reticulum resident protein 60|epididymis secretory protein Li 269|epididymis secretory sperm binding protein Li 93n|glucose regulated protein, 58kDa|phospholipase C-alpha|protein disulfide isomerase-associated 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERp57, check out the ERp57 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERp57: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERp57 (GRP58) Monoclonal Antibody (M01464-1)
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