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Product Info Summary
| SKU: | A05659-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ERN2 Antibody Picoband®
SKU/Catalog Number
A05659-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ERN2 Antibody Picoband® catalog # A05659-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ERN2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05659-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ERN2 recombinant protein (Position: K517-P919).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A05659-2 is reactive to ERN2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
102 kDa
Calculated molecular weight
101272 MW
Background of ERN2
ERN2 induces translational repression through 28S ribosomal RNA cleavage in response to endoplasmic reticulum (ER) stress. This pro-apoptotic appears to play no role in the unfolded-protein response, unlike closely related proteins. Overexpression of ERN2 activates both BiP and CHOP expression, and also leads to the development of programmed cell death. It has been suggested that Ern2 plays a role in multiple facets of the ER stress response in mammalian cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05659-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human HCCT tissue, human HepG2 whole cell
FCM: Jurkat cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ERN2 using anti-ERN2 antibody (A05659-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HCCT tissue lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERN2 antigen affinity purified polyclonal antibody (Catalog # A05659-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERN2 at approximately 102 kDa. The expected band size for ERN2 is at 102 kDa.
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Figure 2. Flow Cytometry analysis of Jurkat cells using anti-ERN2 antibody (A05659-2).
Overlay histogram showing Jurkat cells stained with A05659-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERN2 Antibody (A05659-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ERN2 (Source: Uniprot.org, NCBI)
Gene Name
ERN2
Full Name
Serine/threonine-protein kinase/endoribonuclease IRE2
Weight
101272 MW
Superfamily
protein kinase superfamily
Alternative Names
N-alpha-acetyltransferase 15, NatA auxiliary subunit;Gastric cancer antigen Ga19;N-terminal acetyltransferase;NMDA receptor-regulated protein 1;Protein tubedown-1;Tbdn100;NAA15;GA19, NARG1, NATH, TBDN100; ERN2 IRE1-BETA, IRE1b, IRE2p, hIRE2p endoplasmic reticulum to nucleus signaling 2 serine/threonine-protein kinase/endoribonuclease IRE2|ER to nucleus signalling 2|IRE1 beta|IRE1, S. cerevisiae, homolog of|endoplasmic reticulum to nucleus signalling 2|inositol-requiring 1 beta|inositol-requiring protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERN2, check out the ERN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERN2 Antibody Picoband® (A05659-2)
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