Product Info Summary
SKU: | M01507 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Ephrin type-B receptor 2 EphB2 Monoclonal Antibody
SKU/Catalog Number
M01507
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ephrin type-B receptor 2 EphB2 Monoclonal Antibody catalog # M01507. Tested in ELISA, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ephrin type-B receptor 2 EphB2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01507)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2D12C6
Isotype
IgG
Immunogen
Purified recombinant fragment of EphB2 (aa17-200) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01507 is reactive to EPHB2 in Human
Reconstitution
Calculated molecular weight
117493 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01507 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunofluorescent staining data of EPHB2 using Anti-Ephrin type-B receptor 2 EphB2 Monoclonal Antibody (M01507).
Immunofluorescence (IF) analysis of HeLa (left) and HepG2 (right) cells using EphB2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Click image to see more details
Figure 1. Western blotting validation for Anti-Ephrin type-B receptor 2 EphB2 Monoclonal Antibody M01507
Western Blot (WB) analysis using EphB2 Monoclonal Antibody against truncated EphB2 recombinant protein (1) and extracellular EphB2(aa19-476)-hIgGFc transfected CHO-K1 cell lysate(2).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For EPHB2 (Source: Uniprot.org, NCBI)
Gene Name
EPHB2
Full Name
Ephrin type-B receptor 2
Weight
117493 MW
Superfamily
protein kinase superfamily
Alternative Names
Ephrin type-B receptor 2;2.7.10.1;Developmentally-regulated Eph-related tyrosine kinase;ELK-related tyrosine kinase;EPH tyrosine kinase 3;EPH-like kinase 5;EK5;hEK5;Renal carcinoma antigen NY-REN-47;Tyrosine-protein kinase TYRO5;Tyrosine-protein kinase receptor EPH-3;EphB2/CTF1 EPHB2 BDPLT22, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5 EPH receptor B2 ephrin type-B receptor 2|EPH-like kinase 5|developmentally-regulated Eph-related tyrosine kinase|elk-related tyrosine kinase|eph tyrosine kinase 3|protein-tyrosine kinase HEK5|renal carcinoma NY-REN-47|tyrosine-protein kinase TYRO5|tyrosine-protein kinase receptor EPH-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHB2, check out the EPHB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ephrin type-B receptor 2 EphB2 Monoclonal Antibody (M01507)
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