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Product Info Summary
| SKU: | A04659 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EPHB3 Antibody Picoband®
SKU/Catalog Number
A04659
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPHB3 Antibody Picoband® catalog # A04659. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-EPHB3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04659)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EPHB3 recombinant protein (Position: H275-Q554).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04659 is reactive to EPHB3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
110 kDa
Calculated molecular weight
110.33kDa
Background of EPHB3
Ephrin Receptor EphB3, is also known as human embryo kinase2(HEK2) or Eph-like tyrosine kinase2(ETK2). HEK2, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. HEK2 interacts with 2 ligands of EPH-related kinases(LERKs), namely, LERK2(EFNB1) and LERK5(EFNB2). Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of HEK2 and LERK2 results in reduced kinase activity of HEK2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04659 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A431 whole cell, human Hacat whole cell, rat brain tissue, rat stomach tissue, rat lung tissue, mouse brain tissue, mouse stomach tissue, mouse lung tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EPHB3 using anti-EPHB3 antibody (A04659).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse stomach tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB3 antigen affinity purified polyclonal antibody (Catalog # A04659) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHB3 at approximately 110 kDa. The expected band size for EPHB3 is at 110-130 kDa.
Protein Target Info & Infographic
Gene/Protein Information For EPHB3 (Source: Uniprot.org, NCBI)
Gene Name
EPHB3
Full Name
Ephrin type-B receptor 3
Weight
110.33kDa
Superfamily
protein kinase superfamily
Alternative Names
Agrin; Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90; Agrin C-terminal 22 kDa fragment; C22; AGRN; AGRIN EPHB3 EK2, ETK2, HEK2, TYRO6 EPH receptor B3 ephrin type-B receptor 3|EPH-like tyrosine kinase 2|embryonic kinase 2|human embryo kinase 2|tyrosine-protein kinase TYRO6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHB3, check out the EPHB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPHB3 Antibody Picoband® (A04659)
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