Anti-Eph receptor B3/EPHB3 Antibody Picoband®

Product Name

Anti-Eph receptor B3/EPHB3 Antibody Picoband®

View all EPHB3 Antibodies

SKU/Catalog Number

PA1569

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Eph receptor B3/EPHB3 Antibody catalog # PA1569. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Eph receptor B3/EPHB3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1569)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor B3, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1569 is reactive to EPHB3 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

130 kDa

Calculated molecular weight

110330 MW

Background of EPHB3

Ephrin Receptor EphB3, is also known as human embryo kinase2 (HEK2) or Eph-like tyrosine kinase2 (ETK2). HEK2, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. HEK2 interacts with 2 ligands of EPH-related kinases (LERKs), namely, LERK2 (EFNB1) and LERK5 (EFNB2). Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of HEK2 and LERK2 results in reduced kinase activity of HEK2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1569 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: HELA Cell, A549 Cell
IHC: Human Lung Cancer Tissue

Validation Images & Assay Conditions

Gene/Protein Information For EPHB3 (Source: Uniprot.org, NCBI)

Alternative Names

Ephrin type-B receptor 3;2.7.10.1;EPH-like tyrosine kinase 2;EPH-like kinase 2;Embryonic kinase 2;EK2;hEK2;Tyrosine-protein kinase TYRO6;EPHB3;ETK2, HEK2, TYRO6; EPHB3 EK2, ETK2, HEK2, TYRO6 EPH receptor B3 ephrin type-B receptor 3|EPH-like tyrosine kinase 2|embryonic kinase 2|human embryo kinase 2|tyrosine-protein kinase TYRO6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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