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Product Info Summary
| SKU: | PA1573 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Eph receptor A1/EPHA1 Antibody Picoband®
SKU/Catalog Number
PA1573
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Eph receptor A1/EPHA1 Antibody catalog # PA1573. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Eph receptor A1/EPHA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1573)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor A1, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1573 is reactive to EPHA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
113 kDa
Calculated molecular weight
108127 MW
Background of EPHA1
EPHA1, EPH receptor A1, is a protein that in humans is encoded by the EPHA1 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The EPHA1 gene is mapped to 7q34-q35. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1573 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human Hela whole cell, human Colo320 whole cell, human HepG2 whole cell, human Caco-2 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
IHC: Rat Brain Tissue, Human Colon Cancer Tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Eph receptor A1 using anti-Eph receptor A1 antibody (PA1573).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Colo320 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A1 antigen affinity purified polyclonal antibody (Catalog # PA1573) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A1 at approximately 113 kDa. The expected band size for Eph receptor A1 is at 108 kDa.
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Anti-Eph receptor A1 antibody, PA1573, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-Eph receptor A1 antibody, PA1573, IHC(P)
IHC(P): Human Colon Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For EPHA1 (Source: Uniprot.org, NCBI)
Gene Name
EPHA1
Full Name
Ephrin type-A receptor 1
Weight
108127 MW
Superfamily
protein kinase superfamily
Alternative Names
Ephrin type-A receptor 1;hEpha1;2.7.10.1;EPH tyrosine kinase;EPH tyrosine kinase 1;Erythropoietin-producing hepatoma receptor;Tyrosine-protein kinase receptor EPH;EPHA1;EPH, EPHT, EPHT1; EPHA1 EPH, EPHT, EPHT1 EPH receptor A1 ephrin type-A receptor 1|eph tyrosine kinase 1|erythropoietin-producing hepatoma amplified sequence|erythropoietin-producing hepatoma receptor|hEpha1|oncogene EPH|soluble EPHA1 variant 1|soluble EPHA1 variant 2|tyrosine-protein kinase receptor EPH
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EPHA1, check out the EPHA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EPHA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Eph receptor A1/EPHA1 Antibody Picoband® (PA1573)
Hello CJ!
PA1573 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Başak Büyük,Cemre Aydeğer,Yasemen Adalı et al.The Effect of Topically Applied Boric Acid on Ephrin-Eph Pathway in Wound Treatment: An Experimental Study,14 June 2021,PREPRINT(Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-561841/v1]
Species: Rat
PA1573 usage in article: APP:IHC, SAMPLE:DORSAL TISSUE, DILUTION:NA
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Customer Q&As
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1 Customer Q&As for Anti-Eph receptor A1/EPHA1 Antibody Picoband®
Question
We are currently using anti-Eph receptor A1/EPHA1 antibody PA1573 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The anti-Eph receptor A1/EPHA1 antibody (PA1573) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-31
