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Product Info Summary
| SKU: | M01670 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ENaC beta Monoclonal Antibody
View all epithelial Sodium Channel beta Antibodies
SKU/Catalog Number
M01670
Size
100ug
Form
liquid
Description
Boster Bio Anti-ENaC beta Monoclonal Antibody catalog # M01670. Tested in IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ENaC beta Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01670)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
7B8
Isotype
IgG1
Immunogen
Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~87kDa.
Reactive Species
M01670 is reactive to SCNN1B in Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
62081 MW
Background of epithelial Sodium Channel beta
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01670 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000); IHC (1:150); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Scnn1b using anti-Scnn1b antibody (M01670).
Scnn1b was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Scnn1b Antibody (M01670) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 2. Western blot analysis of Scnn1b using anti-Scnn1b antibody (M01670).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scnn1b antigen affinity purified polyclonal antibody (Catalog # M01670) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Scnn1b.
Protein Target Info & Infographic
Gene/Protein Information For SCNN1B (Source: Uniprot.org, NCBI)
Gene Name
SCNN1B
Full Name
Amiloride-sensitive sodium channel subunit beta
Weight
62081 MW
Superfamily
amiloride-sensitive sodium channel (TC 1.A.6) family
Alternative Names
SCNN1B, Amiloride sensitive sodium channel subunit beta, Amiloride-sensitive sodium channel beta-subunit, Beta ENaC, Beta NaCH, ENaC beta, ENaCB, Epithelial Na(+) channel subunit beta, Epithelial Na+ channel beta subunit, Epithelial Na+ channel subunit beta, Epithelial sodium channel beta 2 subunit, Epithelial sodium channel beta 3 subunit, Nonvoltage gated sodium channel 1 beta subunit, Nonvoltage gated sodium channel 1 subunit beta, Nonvoltage-gated sodium channel 1 beta subunit, SCNEB, SCNN 1B, Sodium channel nonvoltage gated 1 beta (Liddle syndrome), Sodium channel nonvoltage gated 1 beta SCNN1B BESC1, ENaCb, ENaCbeta, LIDLS1, SCNEB sodium channel epithelial 1 subunit beta amiloride-sensitive sodium channel subunit beta|amiloride-sensitive sodium channel subunit beta 1|beta-ENaC|beta-NaCH|epithelial Na(+) channel subunit beta|epithelial sodium channel beta-2 subunit|epithelial sodium channel beta-3 subunit|mutant sodium channel epithelial 1 beta subunit|nasal epithelial sodium channel beta subunit|nonvoltage-gated sodium channel 1 subunit beta|sodium channel epithelial 1 beta subunit|sodium channel, non-voltage-gated 1, beta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCNN1B, check out the SCNN1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCNN1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ENaC beta Monoclonal Antibody (M01670)
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Customer Q&As
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3 Customer Q&As for Anti-ENaC beta Monoclonal Antibody
Question
Is this M01670 anti-ENaC beta Monoclonal antibody reactive to the isotypes of SCNN1B?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
The immunogen of M01670 anti-ENaC beta Monoclonal antibody is Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-24
Question
We are currently using anti-ENaC beta Monoclonal antibody M01670 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
The anti-ENaC beta Monoclonal antibody (M01670) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-04
Question
Do you have a BSA free version of anti-ENaC beta Monoclonal antibody M01670 available?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ENaC beta Monoclonal antibody M01670 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-11
