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Product Info Summary
| SKU: | A10175 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ELOA1 TCEB3 Antibody
SKU/Catalog Number
A10175
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ELOA1 TCEB3 Antibody (Catalog # A10175). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ELOA1 TCEB3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10175)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ELOA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10175 is reactive to TCEB3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
89909 MW
Background of ELOA
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
Aso T., Gene 168:277-278(1996).
Gregory S.G., Nature 441:315-321(2006).
Olsen J.V., Cell 127:635-648(2006)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10175 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells and HUVEC cells, using ELOA1 antibody A10175.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ELOA1 antibody A10175.
Protein Target Info & Infographic
Gene/Protein Information For TCEB3 (Source: Uniprot.org, NCBI)
Gene Name
TCEB3
Full Name
Elongin-A
Weight
89909 MW
Alternative Names
Transcription elongation factor B polypeptide 3;Elongin 110 kDa subunit;Elongin-A;EloA;RNA polymerase II transcription factor SIII subunit A1;SIII p110;TCEB3;MSTP059; ELOA SIII, SIII p110, TCEB3, TCEB3A elongin A elongin-A|RNA polymerase II transcription factor SIII subunit A1|elongin 110 kDa subunit|transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)|transcription elongation factor B alpha subunit|transcription elongation factor B polypeptide 3|transcription elongation factor B subunit 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TCEB3, check out the TCEB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TCEB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ELOA1 TCEB3 Antibody (A10175)
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Customer Q&As
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1 Customer Q&As for Anti-ELOA1 TCEB3 Antibody
Question
I am looking for using your anti-ELOA1 antibody for pausing and recovery of hiv elongation studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.
L. Zhang
Verified customer
Asked: 2015-12-29
Answer
I appreciate your inquiry. This A10175 anti-ELOA1 antibody is validated on jurkat cells, huvec cells, breast carcinoma tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-12-29
