Product Info Summary
SKU: | A06674 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-ELG-1 ATAD5 Antibody
SKU/Catalog Number
A06674
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ELG-1 ATAD5 Antibody (Catalog # A06674). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-ELG-1 ATAD5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06674)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06674 is reactive to ATAD5 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
22745 MW
Background of ATAD5
ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06674 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:3,000 - 1:15,000
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ATAD5 using anti-ATAD5 antibody (A06674).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATAD5 antigen affinity purified polyclonal antibody (Catalog # A06674) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ATAD5.
Protein Target Info & Infographic
Gene/Protein Information For ATAD5 (Source: Uniprot.org, NCBI)
Gene Name
ATAD5
Full Name
ATPase family AAA domain-containing protein 5
Weight
22745 MW
Superfamily
AAA ATPase family
Alternative Names
ELG-1, ELG1, ELG 1, FRAG1, ATP (GTP)-binding protein, ATPase Family AAA Domain-Containing Protein 5, Chromosome fragility associated gene 1 protein, ATAD5, C17orf41 ATAD5 C17orf41, ELG1, FRAG1 ATPase family AAA domain containing 5 ATPase family AAA domain-containing protein 5|chromosome fragility associated gene 1|chromosome fragility-associated gene 1 protein|enhanced level of genomic instability 1 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATAD5, check out the ATAD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATAD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ELG-1 ATAD5 Antibody (A06674)
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Customer Q&As
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5 Customer Q&As for Anti-ELG-1 ATAD5 Antibody
Question
Is a blocking peptide available for product anti-ELG-1 antibody (A06674)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We do provide the blocking peptide for product anti-ELG-1 antibody (A06674). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-30
Question
Do you have a BSA free version of anti-ELG-1 antibody A06674 available?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ELG-1 antibody A06674 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-09
Question
I was wanting to use your anti-ELG-1 antibody for WB for human bone marrow on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human bone marrow identification?
M. Taylor
Verified customer
Asked: 2018-11-14
Answer
You can see on the product datasheet, A06674 anti-ELG-1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-14
Question
I see that the anti-ELG-1 antibody A06674 works with WB, what is the protocol used to produce the result images on the product page?
R. Kulkarni
Verified customer
Asked: 2017-04-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-04-05
Question
We are currently using anti-ELG-1 antibody A06674 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
B. Wu
Verified customer
Asked: 2016-07-05
Answer
The anti-ELG-1 antibody (A06674) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-05