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Product Info Summary
| SKU: | A12947 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-EKI2 ETNK2 Antibody
SKU/Catalog Number
A12947
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-EKI2 ETNK2 Antibody (Catalog # A12947). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EKI2 ETNK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12947)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human EKI2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12947 is reactive to ETNK2 in Human, Mouse
Reconstitution
Calculated molecular weight
44781 MW
Background of ETNK2
Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity By similarity.
Ota T., Nat. Genet. 36:40-45(2004).
Lykidis A., J. Biol. Chem. 276:2174-2179(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12947 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using EKI2 antibody A12947.
Protein Target Info & Infographic
Gene/Protein Information For ETNK2 (Source: Uniprot.org, NCBI)
Gene Name
ETNK2
Full Name
Ethanolamine kinase 2
Weight
44781 MW
Superfamily
choline/ethanolamine kinase family
Alternative Names
Ethanolamine kinase 2;EKI 2;2.7.1.82;Ethanolamine kinase-like protein;ETNK2;EKI2;HMFT1716; ETNK2 EKI2, HMFT1716 ethanolamine kinase 2 ethanolamine kinase 2|ethanolamine kinase-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ETNK2, check out the ETNK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ETNK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EKI2 ETNK2 Antibody (A12947)
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No publications found for A12947
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Customer Q&As
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6 Customer Q&As for Anti-EKI2 ETNK2 Antibody
Question
Is a blocking peptide available for product anti-EKI2 antibody (A12947)?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
We do provide the blocking peptide for product anti-EKI2 antibody (A12947). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-07
Question
Does A12947 anti-EKI2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
It shows on the product datasheet, A12947 anti-EKI2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this A12947 anti-EKI2 antibody reactive to the isotypes of ETNK2?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The immunogen of A12947 anti-EKI2 antibody is Synthesized peptide derived from internal of human EKI2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-10
Question
Does anti-EKI2 antibody A12947 work on monkey WB with right testis?
E. Edwards
Verified customer
Asked: 2019-10-04
Answer
Our lab technicians have not validated anti-EKI2 antibody A12947 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-EKI2 antibody A12947 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey right testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-04
Question
We are currently using anti-EKI2 antibody A12947 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
The anti-EKI2 antibody (A12947) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-19
Question
Will anti-EKI2 antibody A12947 work for WB with hepatoblastoma?
V. Edwards
Verified customer
Asked: 2015-12-04
Answer
According to the expression profile of hepatoblastoma, ETNK2 is highly expressed in hepatoblastoma. So, it is likely that anti-EKI2 antibody A12947 will work for WB with hepatoblastoma.
Boster Scientific Support
Answered: 2015-12-04
