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Product Info Summary
| SKU: | A05520-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-EIF4ENIF1 Antibody Picoband®
SKU/Catalog Number
A05520-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-EIF4ENIF1 Antibody Picoband® catalog # A05520-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-EIF4ENIF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05520-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human EIF4ENIF1 recombinant protein (Position: D13-Q985).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05520-2 is reactive to EIF4ENIF1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
140 kDa
Calculated molecular weight
48608 MW
Background of EIF4ENIF1
Eukaryotic translation initiation factor 4E transporter is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05520-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human HepG2 whole cell, rat brain tissue, rat NRK whole cell, mouse brain tissuemouse NIH/3T3 whole cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EIF4ENIF1 using anti-EIF4ENIF1 antibody (A05520-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse brain tissue lysates.
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4ENIF1 antigen affinity purified polyclonal antibody (Catalog # A05520-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4ENIF1 at approximately 140 kDa. The expected band size for EIF4ENIF1 is at 108,140 kDa.
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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-EIF4ENIF1 antibody (A05520-2).
Overlay histogram showing HL-60 cells stained with A05520-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4ENIF1 Antibody (A05520-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For EIF4ENIF1 (Source: Uniprot.org, NCBI)
Gene Name
EIF4ENIF1
Full Name
Eukaryotic translation initiation factor 4E transporter
Weight
48608 MW
Alternative Names
Spermine oxidase; Polyamine oxidase 1; PAO-1; PAOh1; Smox; Smo EIF4ENIF1 4E-T, Clast4 eukaryotic translation initiation factor 4E nuclear import factor 1 eukaryotic translation initiation factor 4E transporter|2610509L04Rik|4E-transporter|eIF4E transporter
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4ENIF1, check out the EIF4ENIF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4ENIF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EIF4ENIF1 Antibody Picoband® (A05520-2)
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