Click image to see more details
Product Info Summary
| SKU: | A06040 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-eIF3 alpha EIF3J Antibody
SKU/Catalog Number
A06040
Size
100μl
Form
Liquid
Description
Boster Bio Anti-eIF3 alpha EIF3J Antibody catalog # A06040. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF3 alpha EIF3J Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06040)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic Peptide
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06040 is reactive to EIF3J in Human, Mouse, Rat
Applications
A06040 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
29062 MW
Background of EIF3J
Cyclin T2 is a ubiquitously expressed nuclear protein that is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns that contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. Anti-Cyclin T2 Antibody is useful for researchers interested in HIV research, Gene expression, and cell cycle pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using antibody diluted at 1:1000.
Protein Target Info & Infographic
Gene/Protein Information For EIF3J (Source: Uniprot.org, NCBI)
Gene Name
EIF3J
Full Name
Eukaryotic translation initiation factor 3 subunit J
Weight
29062 MW
Superfamily
eIF-3 subunit J family
Alternative Names
eIF3 p35; eIF3-alpha; eIF3j; eIF3-p35; EIF3S1eIF-3-alpha; Eukaryotic translation initiation factor 3 subunit 1; eukaryotic translation initiation factor 3 subunit J; eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD); eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 3, subunit J EIF3J EIF3S1, eIF3-alpha, eIF3-p35 eukaryotic translation initiation factor 3 subunit J eukaryotic translation initiation factor 3 subunit J|eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF3J, check out the EIF3J Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF3J: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF3 alpha EIF3J Antibody (A06040)
Hello CJ!
No publications found for A06040
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-eIF3 alpha EIF3J Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-eIF3 alpha EIF3J Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-eIF3 alpha EIF3J Antibody
Question
Does A06040 anti-eIF3 alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
As indicated on the product datasheet, A06040 anti-eIF3 alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-20
Question
We are currently using anti-eIF3 alpha antibody A06040 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
A. Mitchell
Verified customer
Asked: 2018-07-20
Answer
The anti-eIF3 alpha antibody (A06040) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-20
Question
My question regarding product A06040, anti-eIF3 alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06040 anti-eIF3 alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-04
Question
Here is the WB image, lot number and protocol we used for ovarian carcinoma skeletal muscle using anti-eIF3 alpha antibody A06040. Please let me know if you require anything else.
M. Huang
Verified customer
Asked: 2017-03-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-31
Question
Is there a BSA free version of anti-eIF3 alpha antibody A06040 available?
J. Jones
Verified customer
Asked: 2015-04-08
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-eIF3 alpha antibody A06040 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-04-08
Question
I see that the anti-eIF3 alpha antibody A06040 works with WB, what is the protocol used to produce the result images on the product page?
N. Mitchell
Verified customer
Asked: 2014-03-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-17
