Anti-EI24 Antibody

Product Name

Anti-EI24 Antibody

View all EI24 Antibodies

SKU/Catalog Number

A06183

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-EI24 Antibody (Catalog # A06183). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

EI24 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-EI24 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06183)

Host

Rabbit

Contents

EI24 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

EI24 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human EI24. The immunogen is located within the last 50 amino acids of EI24.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of EI24 are known to exist; this antibody will only detect the larger isoform.

Reactive Species

A06183 is reactive to EI24 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

38965 MW

Background of EI24

The etoposide-induced 24 (EI24) protein is an immediate-early induction target of p53-mediated apoptosis and functions as a tumor suppressor. EI24 contains six transmembrane domains and is localized to the endoplasmic reticulum (ER). It binds to Bcl-2 and may contribute to apoptosis by modulating the activity and function of Bcl-2. EI24 is highly mutated in aggressive breast cancers and its loss in breast cancer tissue correlates with tumor invasiveness. Similar findings in invasive cervical carcinoma suggest that EI24 may be a promising therapeutic target in the treatment of invasive cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06183 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

EI24 antibody can be used for detection of EI24 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For EI24 (Source: Uniprot.org, NCBI)

Alternative Names

EPG4, PIG8, TP53I8, p53-induced gene 8 protein EI24 EPG4, PIG8, TP53I8 EI24 autophagy associated transmembrane protein etoposide-induced protein 2.4 homolog|ectopic P-granules autophagy protein 4 homolog|etoposide induced 2.4|p53-induced gene 8 protein|tumor protein p53 inducible protein 8

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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