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Product Info Summary
| SKU: | A00378-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-EGF Antibody Picoband®
SKU/Catalog Number
A00378-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EGF Antibody Picoband® catalog # A00378-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00378-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00378-1 is reactive to Egf in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
6 kDa
Calculated molecular weight
124126 MW
Background of EGF
EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00378-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA(Cap) , 1-5μg/ml, Rat
Positive Control
IHC: rat kidney tissue, rat small intestine tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EGF using anti-EGF antibody (A00378-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant rat EGF protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF antigen affinity purified polyclonal antibody (Catalog # A00378-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGF at approximately 6KD. The expected band size for EGF is at 6KD.
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Figure 2. IHC analysis of EGF using anti-EGF antibody (A00378-1).
EGF was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EGF Antibody (A00378-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of EGF using anti-EGF antibody (A00378-1).
EGF was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EGF Antibody (A00378-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Sandwich ELISA - Recombinant rat EGF protein standard curve.
Use in combination with reagents from Rat EGF ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0954).
Protein Target Info & Infographic
Gene/Protein Information For Egf (Source: Uniprot.org, NCBI)
Gene Name
Egf
Full Name
Pro-epidermal growth factor
Weight
124126 MW
Alternative Names
Pro-epidermal growth factor;EGF;Epidermal growth factor;Egf; EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Egf, check out the Egf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Egf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EGF Antibody Picoband® (A00378-1)
Hello CJ!
A00378-1 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat
Therapeutic Effect and Mechanism of Oxytropis falcata Gel on Deep Second-Degree Burn in Rats
Venlafaxine-induced adrenergic signaling stimulates Leydig cells steroidogenesis via Nur77 overexpression: a possible role of EGF
Transdermal delivery of human epidermal growth factor facilitated by a peptide chaperon
Biomimetic hydrogel for rapid and scar-free healing of skin wounds inspired by the healing process of oral mucosa
Proton pump inhibitors are not the key for therapying non-steroidal anti-inflammatory drugs-induced small intestinal injury
The effect of a self‐assembling peptide nanofiber scaffold (peptide) when used as a wound dressing for the treatment of deep second degree burns in rats
Therapeutic Effect and Mechanism of Oxytropis falcata Gel on Deep Second-Degree Burn in Rats
Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat
Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat
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Customer Q&As
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16 Customer Q&As for Anti-EGF Antibody Picoband®
Question
Can you help my question with product A00378-1, anti-EGF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00378-1 anti-EGF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-30
Question
I see that the anti-EGF antibody A00378-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-12
Question
I was wanting to use your anti-EGF antibody for WB for rat renal medulla on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat renal medulla identification?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
You can see on the product datasheet, A00378-1 anti-EGF antibody has been validated for ELISA, IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat renal medulla in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-06
Question
Is a blocking peptide available for product anti-EGF antibody (A00378-1)?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
We do provide the blocking peptide for product anti-EGF antibody (A00378-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-21
Question
Do you have a BSA free version of anti-EGF antibody A00378-1 available?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-EGF antibody A00378-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-31
Question
We are currently using anti-EGF antibody A00378-1 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-EGF antibody (A00378-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
Will anti-EGF antibody A00378-1 work for WB with renal medulla?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
According to the expression profile of renal medulla, EGF is highly expressed in renal medulla. So, it is likely that anti-EGF antibody A00378-1 will work for WB with renal medulla.
Boster Scientific Support
Answered: 2019-09-09
Question
We purchased anti-EGF antibody for ELISA on kidney a few months ago. I am using rat, and We intend to use the antibody for WB next. I am looking for examining kidney as well as teratocarcinoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
I looked at the website and datasheets of our anti-EGF antibody and I see that A00378-1 has been tested on rat in both ELISA and WB. Thus A00378-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-10
Question
Is this A00378-1 anti-EGF antibody reactive to the isotypes of EGF?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The immunogen of A00378-1 anti-EGF antibody is E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-12
Question
Our team were well pleased with the WB result of your anti-EGF antibody. However we have observed positive staining in teratocarcinoma membrane using this antibody. Is that expected? Could you tell me where is EGF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
According to literature, teratocarcinoma does express EGF. Generally EGF expresses in membrane. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-06-29
Question
Will A00378-1 anti-EGF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
As indicated on the product datasheet, A00378-1 anti-EGF antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-25
Question
Here is the WB image, lot number and protocol we used for renal medulla using anti-EGF antibody A00378-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for renal medulla using anti-EGF antibody A00378-1. Let me know if you need anything else.
K. Miller
Verified customer
Asked: 2016-12-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-09
Question
We have been able to see staining in rat colon. Any tips? Is anti-EGF antibody supposed to stain colon positively?
C. Li
Verified customer
Asked: 2016-04-04
Answer
From literature colon does express EGF. From Uniprot.org, EGF is expressed in renal medulla, kidney, teratocarcinoma, colon, among other tissues. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-04-04
Question
My question regards to test anti-EGF antibody A00378-1 on rat renal medulla for research purposes, then I may be interested in using anti-EGF antibody A00378-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Zhao
Verified customer
Asked: 2015-07-30
Answer
The products we sell, including anti-EGF antibody A00378-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-07-30
Question
I was wanting to use using your anti-EGF antibody for epidermal growth factor receptor signaling pathway studies. Has this antibody been tested with western blotting on rat kidney tissue? We would like to see some validation images before ordering.
E. Anderson
Verified customer
Asked: 2013-04-17
Answer
We appreciate your inquiry. This A00378-1 anti-EGF antibody is tested on rat kidney tissue, small intestine tissue. It is guaranteed to work for ELISA, IHC, WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-04-17
