Product Info Summary
SKU: | A02277-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-eEF2K Antibody
SKU/Catalog Number
A02277-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-eEF2K Antibody (Catalog# A02277-1). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eEF2K Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02277-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human eEF2K. Immunogen sequence location: 336-385
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02277-1 is reactive to Eef2k in Human, Mouse, Rat
Applications
A02277-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
82144 MW
Background of Eef2k
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from Jurkat cells, using eEF2K Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For Eef2k (Source: Uniprot.org, NCBI)
Gene Name
Eef2k
Full Name
Eukaryotic elongation factor 2 kinase
Weight
82144 MW
Superfamily
protein kinase superfamily
Alternative Names
Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; EC 2.7.11; EC 2.7.11.20; eEF-2 kinase; eEF-2Kcalmodulin-dependent protein kinase III; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; MGC45041 Eef2k|C86191, eEF-2K|eukaryotic elongation factor-2 kinase|eukaryotic elongation factor 2 kinase|calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase|eEF-2 kinase|eukaroytic elongation factor 2 kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Eef2k, check out the Eef2k Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Eef2k: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eEF2K Antibody (A02277-1)
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No publications found for A02277-1
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Customer Q&As
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7 Customer Q&As for Anti-eEF2K Antibody
Question
Is a blocking peptide available for product anti-eEF2K antibody (A02277-1)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We do provide the blocking peptide for product anti-eEF2K antibody (A02277-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-25
Question
We are currently using anti-eEF2K antibody A02277-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The anti-eEF2K antibody (A02277-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-28
Question
Does A02277-1 anti-eEF2K antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
You can see on the product datasheet, A02277-1 anti-eEF2K antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-04
Question
Is there a BSA free version of anti-eEF2K antibody A02277-1 available?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-eEF2K antibody A02277-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-04
Question
I see that the anti-eEF2K antibody A02277-1 works with WB, what is the protocol used to produce the result images on the product page?
P. Wu
Verified customer
Asked: 2018-03-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-eEF2K antibody A02277-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-30
Question
We want to test anti-eEF2K antibody A02277-1 on mouse lymph for research purposes, then I may be interested in using anti-eEF2K antibody A02277-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
W. Carter
Verified customer
Asked: 2014-10-21
Answer
The products we sell, including anti-eEF2K antibody A02277-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-10-21