Anti-EEF2 Monoclonal Antibody

eEF-2 antibody

Boster Bio Anti-EEF2 Monoclonal Antibody catalog # M00830-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00830-1
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-EEF2 Monoclonal Antibody

View all eEF-2 Antibodies

SKU/Catalog Number

M00830-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-EEF2 Monoclonal Antibody catalog # M00830-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EEF2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00830-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADBH-5

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human EEF2 Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00830-1 is reactive to EEF2 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

100 kDa

Calculated molecular weight

95 kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00830-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
FC 1:50

Positive Control

WB: human 293T whole cell, human SiHa whole cell, human Jurkat whole cell, human A549 whole cell, human K562 whole cell, human MCF-7 whole cell, human HEL whole cell, human Caco-2 whole cell
IHC: human prostate cancer tissue, rat ovary tissue

Validation Images & Assay Conditions

Gene/Protein Information For EEF2 (Source: Uniprot.org, NCBI)

Gene Name

EEF2

Full Name

Elongation factor 2

Weight

95 kDa

Superfamily

TRAFAC class translation factor GTPase superfamily

Alternative Names

Prolow-density lipoprotein receptor-related protein 1;LRP-1;Alpha-2-macroglobulin receptor;A2MR;Apolipoprotein E receptor;APOER;CD91;Low-density lipoprotein receptor-related protein 1 85 kDa subunit;LRP-85;Low-density lipoprotein receptor-related protein 1 515 kDa subunit;LRP-515;Low-density lipoprotein receptor-related protein 1 intracellular domain;LRPICD;LRP1;A2MR, APR; EEF2 EEF-2, EF-2, EF2, SCA26 eukaryotic translation elongation factor 2 elongation factor 2|epididymis secretory sperm binding protein|polypeptidyl-tRNA translocase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EEF2, check out the EEF2 Infographic

EEF2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EEF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M00830-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-EEF2 Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-EEF2 Monoclonal Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Anti-EEF2 Monoclonal Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-EEF2 Monoclonal antibody M00830-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-26

Question

Would M00830-1 anti-EEF2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

You can see on the product datasheet, M00830-1 anti-EEF2 Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-05

Question

Is a blocking peptide available for product anti-EEF2 Monoclonal antibody (M00830-1)?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

We do provide the blocking peptide for product anti-EEF2 Monoclonal antibody (M00830-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-30

Question

We are currently using anti-EEF2 Monoclonal antibody M00830-1 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2017-12-01

Answer

The anti-EEF2 Monoclonal antibody (M00830-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-01

Question

I was wanting to use your anti-EEF2 Monoclonal antibody for IP for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?

A. Jones

Verified customer

Asked: 2015-05-07

Answer

It shows on the product datasheet, M00830-1 anti-EEF2 Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-05-07

Question

Would anti-EEF2 Monoclonal antibody M00830-1 work on goat WB with liver?

M. Krishna

Verified customer

Asked: 2014-01-06

Answer

Our lab technicians have not validated anti-EEF2 Monoclonal antibody M00830-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-EEF2 Monoclonal antibody M00830-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-01-06

Order DetailsPrice
M00830-1

100μL

$370
M00830-1-30ul

30μL sample

$99
M00830-1-carrier-free-1ml

Carrier Free

$2590

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
M00830-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.