Click image to see more details
Product Info Summary
| SKU: | M08532 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-EEF1E1 Antibody
SKU/Catalog Number
M08532
Size
80 µl
Description
Boster Bio Anti-EEF1E1 Antibody (Catalog # M08532). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-EEF1E1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08532)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1494CT639.147.31
Isotype
IgG2b,κ
Immunogen
This EEF1E1 antibody is generated from a mouse immunized with a recombinant protein of human EEF1E1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08532 is reactive to EEF1E1 in Human, Mouse
Reconstitution
Calculated molecular weight
19811 Da
Background of EEF1E1
Positive modulator of ATM response to DNA damage.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08532 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000-1:4000
FC: 1:25
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling EEF1E1 with M08532 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Click image to see more details
All lanes : Anti-EEF1E1 Antibody at 1:2000-1:4000 dilution
Lane 1: HepG2 whole cell lysates
Lane 2: human testis lysates
Lane 3: A549 whole cell lysates
Lane 4: A431 whole cell lysates
Lane 5: Jurkat whole cell lysates
Lane 6: mouse brain lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 20 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Overlay histogram showing Hela cells stained with M08532 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M08532, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For EEF1E1 (Source: Uniprot.org, NCBI)
Gene Name
EEF1E1
Full Name
Eukaryotic translation elongation factor 1 epsilon-1
Weight
19811 Da
Alternative Names
Eukaryotic translation elongation factor 1 epsilon-1, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Elongation factor p18, Multisynthase complex auxiliary component p18, EEF1E1, AIMP3, P18 EEF1E1 AIMP3, P18 eukaryotic translation elongation factor 1 epsilon 1 eukaryotic translation elongation factor 1 epsilon-1|ARS-interacting multifunctional protein 3|aminoacyl tRNA synthetase complex-interacting multifunctional protein 3|multisynthase complex auxiliary component p18|p18 component of aminoacyl-tRNA synthetase complex
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EEF1E1, check out the EEF1E1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EEF1E1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EEF1E1 Antibody (M08532)
Hello CJ!
No publications found for M08532
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-EEF1E1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-EEF1E1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
