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Product Info Summary
| SKU: | A03187-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-E74 like factor 1/ELF1 Antibody Picoband®
View all E74 like factor 1 Antibodies
SKU/Catalog Number
A03187-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-E74 like factor 1/ELF1 Antibody Picoband® catalog # A03187-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E74 like factor 1/ELF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03187-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human E74 like factor 1, which shares 81.6% amino acid (aa) sequence identity with both mouse and rat E74 like factor 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03187-1 is reactive to ELF1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
67.498kDa
Background of E74 like factor 1
E74-like factor 1 (ets domain transcription factor) is a protein that in humans is encoded by the ELF1 gene. It is mapped to chromosome 13q13. This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03187-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, human COLO-320 whole cell, human A549 whole cell, human PANC-1 whole cell, Human A431 whole cell, human Jurkat whole cell, human 293T whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of E74 like factor 1 using anti-E74 like factor 1 antibody (A03187-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: Human A431 whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: human 293T whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E74 like factor 1 antigen affinity purified polyclonal antibody (Catalog # A03187-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E74 like factor 1 at approximately 75KD. The expected band size for E74 like factor 1 is at 67KD.
Protein Target Info & Infographic
Gene/Protein Information For ELF1 (Source: Uniprot.org, NCBI)
Gene Name
ELF1
Full Name
ETS-related transcription factor Elf-1
Weight
67.498kDa
Superfamily
ETS family
Alternative Names
ETS-related transcription factor Elf-1; E74-like factor 1; ELF1 ELF1 EFTUD1, RIA1 E74 like ETS transcription factor 1 ETS-related transcription factor Elf-1|E74-like factor 1 (ets domain transcription factor)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELF1, check out the ELF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E74 like factor 1/ELF1 Antibody Picoband® (A03187-1)
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Customer Q&As
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3 Customer Q&As for Anti-E74 like factor 1/ELF1 Antibody Picoband®
Question
We are currently using anti-E74 like factor 1/ELF1 antibody A03187-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The anti-E74 like factor 1/ELF1 antibody (A03187-1) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-18
Question
Do you have a BSA free version of anti-E74 like factor 1/ELF1 antibody A03187-1 available?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-E74 like factor 1/ELF1 antibody A03187-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-20
Question
Will anti-E74 like factor 1/ELF1 antibody A03187-1 work for WB with lymph?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
According to the expression profile of lymph, ELF1 is highly expressed in lymph. So, it is likely that anti-E74 like factor 1/ELF1 antibody A03187-1 will work for WB with lymph.
Boster Scientific Support
Answered: 2018-07-20
