Anti-Phospho-E2F-1 S364 Antibody

Product Name

Anti-Phospho-E2F-1 S364 Antibody

View all E2F-1 Antibodies

SKU/Catalog Number

P00257

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-E2F-1 S364 Antibody (Catalog # P00257). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Phospho-E2F-1 S364 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00257)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human E2F-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P00257 is reactive to E2F1 in Human, Mouse

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

46920 MW

Background of E2F-1

E2F-1 (also known as transcription factor E2F-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains conserved through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and two other members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis as well as it can block adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Anti-E2F-1 pS364 Antibody is useful for researchers involved in transcription factor and DNA binding research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P00257 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IHC: 2 mg/ml - 20 µg/ml
WB: 1:250 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For E2F1 (Source: Uniprot.org, NCBI)

Alternative Names

E2F-1 pS364, E2F 1, transcription factor E2F1, E2F1, E2F transcription factor 1, PBR 3, PBR3, Retinoblastoma binding protein 3, RBBP-3, pRB-binding protein E2F-1, Retinoblastoma-associated protein 1, RBAP-1 E2F1 E2F-1, RBAP1, RBBP3, RBP3 E2F transcription factor 1 transcription factor E2F1|PBR3|PRB-binding protein E2F-1|RBAP-1|RBBP-3|retinoblastoma-associated protein 1|retinoblastoma-binding protein 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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