Anti-Dystrophin Dmd Antibody (Monoclonal, MANDYS8)

Product Name

Anti-Dystrophin Dmd Antibody (Monoclonal, MANDYS8)

View all Dystrophin Antibodies

SKU/Catalog Number

MA1037

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Dystrophin Dmd Antibody (Monoclonal, MANDYS8) catalog # MA1037. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Dystrophin Dmd Antibody (Monoclonal, MANDYS8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1037)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

MANDYS8

Isotype

Mouse IgG2b

Immunogen

Recombinant human dystrophin fragment.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1037 is reactive to DMD in Human, Mouse, Rabbit, Rat

Applications

MA1037 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

426 kDa

Calculated molecular weight

425828 MW

Background of Dystrophin

Dystrophin (DMD) gene has 79 exons spanning at least 2,300 kb (2.3 Mb). The C terminus of the dystrophin protein is encoded by a highly conserved, alternatively spliced region of the gene. beta-dystroglycan binding activity is expressed by the dystrophin fragment spanning amino acids 3026-3345 containing the ZZ domain. DMD transcript is formed by at least 60 exons; the first half of the transcript is formed by a minimum of 33 exons spanning nearly 1000 kb, and the remaining portion has at least 27 exons that may spread over a similar distance. Dystrophin gene is expressed at a higher level in primary cultures of neuronal cells than in astro-glial cells derived from adult mouse brain. overexpression of dystrophin prevents the development of the abnormal mechanical properties associated with dystrophic muscle without causing deleterious side effects.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2 ml of PBS buffer will yield a concentration of 500 ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 1-2μg/ml, Human, mouse, rabbit, rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Dystrophin using anti-Dystrophin antibody (MA1037).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Dystrophin antigen affinity purified monoclonal antibody (Catalog # MA1037) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Dystrophin at approximately 426 kDa. The expected band size for Dystrophin is at 426 kDa.

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Figure 2. IHC analysis of Dystrophin using anti-Dystrophin antibody (MA1037).
Dystrophin was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Dystrophin Antibody (MA1037) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Gene/Protein Information For DMD (Source: Uniprot.org, NCBI)

Alternative Names

BMDDXS272; CMD3B; DXS142; DXS164; DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142; dystrophin DMD BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85 dystrophin dystrophin|truncated dystrophin

For more info on DMD, check out the DMD Infographic

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In this infographic, you will see the following information for DMD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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