Click image to see more details
Product Info Summary
| SKU: | A06890-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Dynamin 3/DNM3 Antibody Picoband®
SKU/Catalog Number
A06890-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dynamin 3/DNM3 Antibody Picoband® catalog # A06890-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dynamin 3/DNM3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06890-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DNM3 recombinant protein (Position: N295-N712).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06890-2 is reactive to DNM3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
110 kDa
Calculated molecular weight
87148 MW
Background of Dynamin 3
Dynamin-3 is a protein that in humans is encoded by the DNM3 gene. This gene encodes a member of a family of guanosine triphosphate (GTP)-binding proteins that associate with microtubules and are involved in vesicular transport. The encoded protein functions in the development of megakaryocytes. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06890-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: mouse brain tissue, rat brain tissue
FCM: C6 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Dynamin 3/DNM3 using anti-Dynamin 3/DNM3 antibody (A06890-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dynamin 3/DNM3 antigen affinity purified polyclonal antibody (Catalog # A06890-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dynamin 3/DNM3 at approximately 110 kDa. The expected band size for Dynamin 3/DNM3 is at 98 kDa.
Click image to see more details
Figure 2. IHC analysis of Dynamin 3/DNM3 using anti-Dynamin 3/DNM3 antibody (A06890-2).
Dynamin 3/DNM3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dynamin 3/DNM3 Antibody (A06890-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Dynamin 3/DNM3 using anti-Dynamin 3/DNM3 antibody (A06890-2).
Dynamin 3/DNM3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dynamin 3/DNM3 Antibody (A06890-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of C6 cells using anti-Dynamin 3/DNM3 antibody (A06890-2).
Overlay histogram showing C6 cells stained with A06890-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dynamin 3/DNM3 Antibody (A06890-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DNM3 (Source: Uniprot.org, NCBI)
Gene Name
DNM3
Full Name
Dynamin-3
Weight
87148 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
Disintegrin and metalloproteinase domain-containing protein 28;ADAM 28;3.4.24.-;Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II;eMDC II;Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L;MDC-L;ADAM28;ADAM23, MDCL; DNM3 Dyna III dynamin 3 dynamin-3|T-dynamin|dynamin family member|dynamin, testicular
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNM3, check out the DNM3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dynamin 3/DNM3 Antibody Picoband® (A06890-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Dynamin 3/DNM3 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Dynamin 3/DNM3 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
