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Product Info Summary
| SKU: | A02404-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-DVL2 Antibody Picoband®
View all Dishevelled-2 Antibodies
SKU/Catalog Number
A02404-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DVL2 Antibody Picoband® catalog # A02404-4. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DVL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02404-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human DVL2 recombinant protein (Position: L89-Y576). Human DVL2 shares 97.3% amino acid (aa) sequence identity with mouse DVL2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02404-4 is reactive to DVL2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
85 kDa
Calculated molecular weight
79 kDa
Background of Dishevelled-2
Segment polarity protein dishevelled homolog DVL-2 is a protein that in humans is encoded by the DVL2 gene. This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02404-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human MCF-7 whole cell, human A431 whole cell, human Hela whole cell, rat thymus tissue, rat C6 whole cell, mouse thymus tissue, mouse NIH/3T3 whole cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DVL2 using anti-DVL2 antibody (A02404-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DVL2 antigen affinity purified polyclonal antibody (Catalog # A02404-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DVL2 at approximately 85 kDa. The expected band size for DVL2 is at 79 kDa.
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Figure 2. Flow Cytometry analysis of A431 cells using anti-DVL2 antibody (A02404-4).
Overlay histogram showing A431 cells stained with A02404-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DVL2 Antibody (A02404-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DVL2 (Source: Uniprot.org, NCBI)
Gene Name
DVL2
Full Name
Segment polarity protein dishevelled homolog DVL-2
Weight
79 kDa
Superfamily
DSH family
Alternative Names
dishevelled 2 (homologous to Drosophila dsh); dishevelled, dsh homolog 2 (Drosophila); Dishevelled2; Dishevelled-2; DSH homolog 2; DVL2; segment polarity protein dishevelled homolog DVL-2 DVL2 dishevelled segment polarity protein 2 segment polarity protein dishevelled homolog DVL-2|dishevelled 2 (homologous to Drosophila dsh)|dishevelled, dsh homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DVL2, check out the DVL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DVL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DVL2 Antibody Picoband® (A02404-4)
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