Click image to see more details
Product Info Summary
| SKU: | A01035-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-DUP/CDT1 Antibody Picoband®
SKU/Catalog Number
A01035-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DUP/CDT1 Antibody Picoband® catalog # A01035-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DUP/CDT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01035-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DUP/CDT1 recombinant protein (Position: R19-L357).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01035-2 is reactive to CDT1 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
61.51kDa
Background of Cdt1
CDT1 (Chromatin licensing and DNA replication factor 1) is a protein that in humans is encoded by the CDT1 gene. The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01035-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse
ELISA, 0.1-0.5 μg/ml/ml, Human
Positive Control
WB: human MCF-7 whole cell, human Hela whole cell, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of DUP/CDT1 using anti-DUP/CDT1 antibody (A01035-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUP/CDT1 antigen affinity purified polyclonal antibody (Catalog # A01035-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DUP/CDT1 at approximately 60 kDa. The expected band size for DUP/CDT1 is at 60 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CDT1 (Source: Uniprot.org, NCBI)
Gene Name
CDT1
Full Name
DNA replication factor Cdt1
Weight
61.51kDa
Superfamily
Cdt1 family
Alternative Names
Lumican; Keratan sulfate proteoglycan lumican; KSPG lumican; LUM; LDC; SLRR2D; Cdt1|2610318F11Rik, AW545653, C76791, DUP, R, Ris2|chromatin licensing and DNA replication factor 1|DNA replication factor Cdt1|double parked homolog|retroviral insertion site 2 protein|retroviral integration site 1|retroviral integration site 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDT1, check out the CDT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DUP/CDT1 Antibody Picoband® (A01035-2)
Hello CJ!
No publications found for A01035-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-DUP/CDT1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-DUP/CDT1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
