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Product Info Summary
| SKU: | M05530 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Drebrin DBN1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05530
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Drebrin DBN1 Rabbit Monoclonal Antibody catalog # M05530. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Drebrin DBN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05530)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABDC-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Drebrin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05530 is reactive to DBN1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated molecular weight
71429 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05530 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DBN1 using anti-DBN1 antibody (M05530).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBN1 antigen affinity purified polyclonal antibody (Catalog # M05530) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBN1 at approximately 120 kDa. The expected band size for DBN1 is at 71 kDa.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
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Western blot analysis of Drebrin expression in (1) HeLa cell lysate; (2) PC-12 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DBN1 (Source: Uniprot.org, NCBI)
Gene Name
DBN1
Full Name
Drebrin
Weight
71429 MW
Alternative Names
Drebrin;Developmentally-regulated brain protein;DBN1;D0S117E; DBN1 D0S117E drebrin 1 drebrin|developmentally-regulated brain protein|drebrin A|drebrin E|drebrin E2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DBN1, check out the DBN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DBN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Drebrin DBN1 Rabbit Monoclonal Antibody (M05530)
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