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Product Info Summary
| SKU: | A01110 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Dopamine beta Hydroxylase/DBH Antibody Picoband®
View all Dopamine beta-Hydroxylase Antibodies
SKU/Catalog Number
A01110
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dopamine beta Hydroxylase/DBH Antibody Picoband® catalog # A01110. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dopamine beta Hydroxylase/DBH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01110)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Dopamine beta Hydroxylase/DBH recombinant protein (Position: S40-N545).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01110 is reactive to DBH in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
51735 MW
Background of Dopamine beta-Hydroxylase
Dopamine beta-hydroxylase (DBH), also known as dopamine beta-monooxygenase, is an enzyme (EC1.14.17.1) that in humans is encoded by the DBH gene. Dopamine beta-hydroxylase catalyzes the chemical reaction. It is mapped to 9q34.2. The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01110 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human Jurkat whole cell, human U87 whole cell, human U20S whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DBH using anti-DBH antibody (A01110).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBH antigen affinity purified polyclonal antibody (Catalog # A01110) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for DBH at approximately 75 kDa. The expected band size for DBH is at 70 kDa.
Protein Target Info & Infographic
Gene/Protein Information For DBH (Source: Uniprot.org, NCBI)
Gene Name
DBH
Full Name
Dopamine beta-hydroxylase
Weight
51735 MW
Superfamily
copper type II ascorbate-dependent monooxygenase family
Alternative Names
Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; DBH DBH DBM, ORTHYP1 dopamine beta-hydroxylase dopamine beta-hydroxylase|dopamine beta-hydroxylase (dopamine beta-monooxygenase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DBH, check out the DBH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DBH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dopamine beta Hydroxylase/DBH Antibody Picoband® (A01110)
Hello CJ!
A01110 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ren S,Chen M,Yang L,Liu Z.5-Hydroxytryptamine and Dopamine Neurons in the Cerebellum of the New-Hatching Yangtze Alligator Alligator sinensis.Anat Rec(Hoboken).2019 Jun;302(6):861-868.doi:10.1002/ar.23982.Epub 2018 Nov 13.PMID:30315688.
Species: Alligators
A01110 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
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Customer Q&As
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1 Customer Q&As for Anti-Dopamine beta Hydroxylase/DBH Antibody Picoband®
Question
We are currently using anti-Dopamine beta Hydroxylase/DBH antibody A01110 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
C. Jha
Verified customer
Asked: 2014-01-14
Answer
The anti-Dopamine beta Hydroxylase/DBH antibody (A01110) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-01-14
