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Product Info Summary
| SKU: | A01771 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-DOCK8 Antibody
View all dedicator of cytokinesis 8 Antibodies
SKU/Catalog Number
A01771
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DOCK8 Antibody (Catalog # A01771). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DOCK8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-DOCK8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01771)
Host
Rabbit
Contents
DOCK8 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DOCK8 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DOCK8. The immunogen is located within the last 50 amino acids of DOCK8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist.
Reactive Species
A01771 is reactive to DOCK8 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
238529 MW
Background of dedicator of cytokinesis 8
The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01771 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DOCK8 in EL4 cell lysate with DOCK8 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of DOCK8 in human spleen tissue with DOCK8 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For DOCK8 (Source: Uniprot.org, NCBI)
Gene Name
DOCK8
Full Name
Dedicator of cytokinesis protein 8
Weight
238529 MW
Superfamily
DOCK family
Alternative Names
MRD2, ZIR8, HEL-205, Dedicator of cytokinesis protein 8 Dock8|1200017A24Rik, 5830472H07Rik, A130095G14Rik, AI461977|dedicator of cytokinesis 8|dedicator of cytokinesis protein 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DOCK8, check out the DOCK8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DOCK8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DOCK8 Antibody (A01771)
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