Anti-DNMT3B Antibody Picoband®

DNMT3B antibody

Boster Bio Anti-DNMT3B Antibody Picoband® catalog # A00319-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00319-2
Size: 100 μg/vial
Reactive Species: Human, Monkey
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

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Product Name

Anti-DNMT3B Antibody Picoband®

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SKU/Catalog Number

A00319-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DNMT3B Antibody Picoband® catalog # A00319-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-DNMT3B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00319-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human DNMT3B recombinant protein (Position: K232-K268).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00319-2 is reactive to DNMT3B in Human, Monkey

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

96 kDa

Calculated molecular weight

29041 MW

Background of DNMT3B

DNA (cytosine-5-)-methyltransferase 3 beta, is an enzyme that in humans in encoded by the DNMT3B gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00319-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Monkey
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Positive Control

WB: human Hela whole cell, monkey COS-7 whole cell, human K562 whole cell
FCM: SiHa cell

Validation Images & Assay Conditions

Gene/Protein Information For DNMT3B (Source: Uniprot.org, NCBI)

Gene Name

DNMT3B

Full Name

DNA (cytosine-5)-methyltransferase 3B

Weight

29041 MW

Superfamily

class I-like SAM-binding methyltransferase superfamily

Alternative Names

Copper chaperone for superoxide dismutase;Superoxide dismutase copper chaperone;CCS; DNMT3B ICF, ICF1, M.HsaIIIB DNA methyltransferase 3 beta DNA (cytosine-5)-methyltransferase 3B|DNA (cytosine-5-)-methyltransferase 3 beta|DNA MTase HsaIIIB|DNA cytosine-5--methyltransferase 3 beta|DNA methyltransferase HsaIIIB

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DNMT3B, check out the DNMT3B Infographic

DNMT3B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DNMT3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00319-2

100μg

$370
A00319-2-10ug

10μg sample (liquid)

$99
A00319-2-Biotin

100 μg Biotin conjugated

$570
A00319-2-Cy3

100 μg Cy3 conjugated

$570
A00319-2-Dylight488

100 μg Dylight488 conjugated

$570
A00319-2-Dylight550

100 μg Dylight550 conjugated

$570
A00319-2-Dylight594

100 μg Dylight594 conjugated

$570
A00319-2-FITC

100 μg FITC conjugated

$570
A00319-2-HRP

100 μg HRP conjugated

$570
A00319-2-APC

100 μg APC conjugated

$670
A00319-2-PE

100 μg PE conjugated

$670
A00319-2-iFluor647

100 μg iFluor647 conjugated

$670
A00319-2-carrier-free

Carrier Free

$370

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A00319-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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