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Product Info Summary
| SKU: | A00645-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DNA-PKCS PRKDC Antibody
SKU/Catalog Number
A00645-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DNA-PKCS PRKDC Antibody catalog # A00645-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA-PKCS PRKDC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00645-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DNA-PK. AA range:4061-4110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00645-1 is reactive to PRKDC in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
469089 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00645-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using DNA-PKCS Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using DNA-PKCS Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PRKDC (Source: Uniprot.org, NCBI)
Gene Name
PRKDC
Full Name
DNA-dependent protein kinase catalytic subunit
Weight
469089 MW
Superfamily
PI3/PI4-kinase family
Alternative Names
DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNA-PKcs;2.7.11.1;DNPK1;p460;PRKDC;HYRC, HYRC1; PRKDC DNA-PKC, DNA-PKcs, DNAPK, DNAPKc, DNPK1, HYRC, HYRC1, IMD26, XRCC7, p350 protein kinase, DNA-activated, catalytic subunit DNA-dependent protein kinase catalytic subunit|DNA-PK catalytic subunit|hyper-radiosensitivity of murine scid mutation, complementing 1|p460|protein kinase, DNA-activated, catalytic polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKDC, check out the PRKDC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKDC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA-PKCS PRKDC Antibody (A00645-1)
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No publications found for A00645-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-DNA-PKCS PRKDC Antibody
Question
Please see the WB image, lot number and protocol we used for liver using anti-DNA-PKCS antibody A00645-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-20
Question
I am interested in to test anti-DNA-PKCS antibody A00645-1 on mouse liver for research purposes, then I may be interested in using anti-DNA-PKCS antibody A00645-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The products we sell, including anti-DNA-PKCS antibody A00645-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-05
Question
Is a blocking peptide available for product anti-DNA-PKCS antibody (A00645-1)?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We do provide the blocking peptide for product anti-DNA-PKCS antibody (A00645-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-18
Question
Will anti-DNA-PKCS antibody A00645-1 work on horse WB with embryonic kidney?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not validated anti-DNA-PKCS antibody A00645-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-DNA-PKCS antibody A00645-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse embryonic kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
Does anti-DNA-PKCS antibody A00645-1 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
According to the expression profile of liver, PRKDC is highly expressed in liver. So, it is likely that anti-DNA-PKCS antibody A00645-1 will work for WB with liver.
Boster Scientific Support
Answered: 2019-08-22
Question
Would A00645-1 anti-DNA-PKCS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
As indicated on the product datasheet, A00645-1 anti-DNA-PKCS antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-17
Question
I see that the anti-DNA-PKCS antibody A00645-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-30
Question
My question regarding product A00645-1, anti-DNA-PKCS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00645-1 anti-DNA-PKCS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-26
Question
We were satisfied with the WB result of your anti-DNA-PKCS antibody. However we have observed positive staining in placenta nucleus using this antibody. Is that expected? Could you tell me where is PRKDC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
From what I have seen in literature, placenta does express PRKDC. Generally PRKDC expresses in nucleus. Regarding which tissues have PRKDC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2507541, 2247066
Cervix carcinoma, Pubmed ID: 7671312, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal lung, Pubmed ID: 7594449
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7638222
Boster Scientific Support
Answered: 2019-02-05
Question
Is this A00645-1 anti-DNA-PKCS antibody reactive to the isotypes of PRKDC?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
The immunogen of A00645-1 anti-DNA-PKCS antibody is Synthesized peptide derived from the C-terminal region of human DNA-PKCS. at AA rangle: 4030-4110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-29
Question
We have been able to see staining in human brain. Are there any suggestions? Is anti-DNA-PKCS antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
Based on literature brain does express PRKDC. Based on Uniprot.org, PRKDC is expressed in kidney, cervix carcinoma, placenta, fetal lung, brain, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PRKDC expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2507541, 2247066
Cervix carcinoma, Pubmed ID: 7671312, 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal lung, Pubmed ID: 7594449
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7638222
Boster Scientific Support
Answered: 2017-10-26
Question
I was wanting to use your anti-DNA-PKCS antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
As indicated on the product datasheet, A00645-1 anti-DNA-PKCS antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-DNA-PKCS antibody A00645-1. Let me know if you need anything else.
H. Miller
Verified customer
Asked: 2017-01-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-10
Question
We are currently using anti-DNA-PKCS antibody A00645-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
B. Walker
Verified customer
Asked: 2015-11-20
Answer
The anti-DNA-PKCS antibody (A00645-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-20
Question
My question regards using your anti-DNA-PKCS antibody for cellular protein modification process studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
S. Parker
Verified customer
Asked: 2015-02-10
Answer
We appreciate your inquiry. This A00645-1 anti-DNA-PKCS antibody is tested on hela cells. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-02-10
Question
Is there a BSA free version of anti-DNA-PKCS antibody A00645-1 available?
R. Huang
Verified customer
Asked: 2014-12-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DNA-PKCS antibody A00645-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-12-17
