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Product Info Summary
| SKU: | RP1083 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-DMRT1 Antibody Picoband®
SKU/Catalog Number
RP1083
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DMRT1 Antibody catalog # RP1083. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DMRT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1083)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DMRT1, different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1083 is reactive to DMRT1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39473 MW
Background of DMRT1
Doublesex and mab-3 related transcription factor 1, also known as DMRT1, is a protein which in humans is encoded by the DMRT1 gene. This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1083 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat testis tissue, mouse testis tissue
IHC: mouse testis tissue, rat testis tissue, human testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DMRT1 using anti-DMRT1 antibody (RP1083).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMRT1 antigen affinity purified polyclonal antibody (Catalog # RP1083) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DMRT1 at approximately 39KD. The expected band size for DMRT1 is at 39KD.
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Figure 2. IHC analysis of DMRT1 using anti-DMRT1 antibody (RP1083).
DMRT1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DMRT1 Antibody (RP1083) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of DMRT1 using anti-DMRT1 antibody (RP1083).
DMRT1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DMRT1 Antibody (RP1083) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of DMRT1 using anti-DMRT1 antibody (RP1083).
DMRT1 was detected in paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DMRT1 Antibody (RP1083) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For DMRT1 (Source: Uniprot.org, NCBI)
Gene Name
DMRT1
Full Name
Doublesex- and mab-3-related transcription factor 1
Weight
39473 MW
Superfamily
DMRT family
Alternative Names
Doublesex- and mab-3-related transcription factor 1;DM domain expressed in testis protein 1;DMRT1;DMT1; DMRT1 CT154, DMT1 doublesex and mab-3 related transcription factor 1 doublesex- and mab-3-related transcription factor 1|DM domain expressed in testis 1|DM domain expressed in testis protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DMRT1, check out the DMRT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DMRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DMRT1 Antibody Picoband® (RP1083)
Hello CJ!
RP1083 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tai S,Zheng Q,Zhai S,Cai T,Xu L,Yang L,Jiao L,Zhang C.Alpha-Lipoic Acid Mediates Clearance of Iron Accumulation by Regulating Iron Metabolism in a Parkinson‘s Disease Model Induced by 6-OHDA.Front Neurosci. 2020 Jun 25;14:612.doi:10.3389/fnins.2020.
Species: Rat
RP1083 usage in article: APP:WB, SAMPLE:PC12 CELLS AND SN TISSUE, DILUTION:NA
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Customer Q&As
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6 Customer Q&As for Anti-DMRT1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-DMRT1 antibody (RP1083)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do provide the blocking peptide for product anti-DMRT1 antibody (RP1083). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-23
Question
Is this RP1083 anti-DMRT1 antibody reactive to the isotypes of DMRT1?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The immunogen of RP1083 anti-DMRT1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human DMRT1 (98-128aa RDCQCKKCNLIAERQRVMAAQVALRRQQAQE), different from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-17
Question
Does anti-DMRT1 antibody RP1083 work on monkey WB with testis?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Our lab technicians have not tested anti-DMRT1 antibody RP1083 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-DMRT1 antibody RP1083 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-DMRT1 antibody RP1083 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The anti-DMRT1 antibody (RP1083) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-DMRT1 antibody RP1083. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
I was wanting to use to test anti-DMRT1 antibody RP1083 on mouse brain for research purposes, then I may be interested in using anti-DMRT1 antibody RP1083 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
The products we sell, including anti-DMRT1 antibody RP1083, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-29
