Anti-DHX29 Antibody Picoband™

Product Name

Anti-DHX29 Antibody Picoband™

View all DHX29 Antibodies

SKU/Catalog Number

A07866

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-DHX29 Antibody Picoband™ catalog # A07866. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-DHX29 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07866)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human DHX29 recombinant protein (Position: R56-K1343).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07866 is reactive to DHX29 in Human

Applications

A07866 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

155 kDa

Calculated molecular weight

49229 MW

Background of DHX29

DExH-box helicase 29 (DHX29) is a 155 kDa protein that in humans is encoded by the DHX29 gene. This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of DHX29 using anti-DHX29 antibody (A07866).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX29 antigen affinity purified polyclonal antibody (Catalog # A07866) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX29 at approximately 155 kDa. The expected band size for DHX29 is at 155 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-DHX29 antibody (A07866).
Overlay histogram showing 293T cells stained with A07866 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHX29 Antibody (A07866, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For DHX29 (Source: Uniprot.org, NCBI)

Alternative Names

ATP-dependent RNA helicase DHX29; DDX29; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 29; DEAH (Asp-Glu-Ala-His) box polypeptide 29; DEAH box protein 29; EC 3.6.1; EC 3.6.4.13; FLJ21492; Nucleic acid helicase DDXx DHX29 DDX29 DExH-box helicase 29 ATP-dependent RNA helicase DHX29|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 29|DEAH (Asp-Glu-Ala-His) box polypeptide 29|DEAH box protein 29|DEAH-box helicase 29|nucleic acid helicase DDXx

For more info on DHX29, check out the DHX29 Infographic

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In this infographic, you will see the following information for DHX29: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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